复合酶对不同类型的烟梗物料酶解效果的比较  

Comparison of hydrolysis effects on different kinds of tobacco stem materials treated by compound enzymes

作　　者：施林燕[1] 于兴伟[1] 许赣荣[1] 汤朝起[2] 金永明[2] 

机构地区：[1]江南大学生物工程学院,无锡214122 [2]上海烟草集团有限责任公司,上海200082

出　　处：《工业微生物》2012年第6期58-62,共5页Industrial Microbiology

摘　　要：在单一酶酶解试验的基础上,进行复合酶酶解试验。采用不同用量的复合酶分别对不同类型的烟梗物料(完整烟梗、烟梗丝、烟梗粉末)进行酶解,通过检测酶解前后果胶酸、还原糖、淀粉等物质的量来判断酶解效果。结果表明:在同一料水比条件下酶解烟梗丝、烟梗粉末及完整烟梗后,以复合酶2酶解烟梗丝分解果胶质的效果最好,其果胶质的降解率为19.85%;以复合酶3酶解烟梗丝,其还原糖含量增加率最大为36.18%;以复合酶3降解烟梗粉末中的淀粉效果最好,其降解率达到37.14%。实验结果表明,从烟梗物料的形态来看,烟梗丝及烟梗粉末的酶解效果较理想,而对形体较大的完整烟梗的酶解效果较差。Based on experiment of enzymatic hydrolysis of cut-stems by using single enzyme, the intact tobacco stem, cut- stems and stem powders were hydrolyzed by compound enzyme preparations. The contents of pectin, reducing sugar, starch and so on were detected to evaluate the effects of enzyme-hydrolysis. The analytical results showed that in the same ratio of material to water, the compound enzyme No. 2 hydrolyzed mostly the pectin of the cut-stems with the highest degra- dation rate of 19.85% ; the cut-stems were hydrolyzed by the compound enzyme No. 3 with the highest reducing sugar of 36.18% ; and the compound enzyme No. 3 hydrolyzed mostly the starch of the stem powder with the highest degradation rate of 37.14%. In terms of particle sizes of the tobacco stems, the effects of enzyme-hydrolysis on cut stem, stem powder were better than that on intact stem.

关 键 词：酶 烟梗丝 烟梗粉末 烟梗 果胶质 还原糖 

分 类 号：Q55[生物学—生物化学]