超高效液相色谱法测定芝麻油中芝麻酚含量  

Determination of sesamol in sesame oil by ultra performance liquid chromatography

作　　者：冯楠[1] 吴颖[2] 路勇[1] 姜洁[1] 谢文东[1] 张慧[2] 

机构地区：[1]北京市食品安全监控中心,北京100041 [2]北京市产品质量监督检验所,北京100029

出　　处：《食品工业科技》2012年第24期66-67,70,共3页Science and Technology of Food Industry

摘　　要：目的:建立芝麻油样品中芝麻酚含量的超高效液相色谱(UPLC)测定方法。方法:样品经90%乙腈水提取后,用水定容,进行UPLC定量分析。实验采用BEH C18色谱柱,以水和乙腈为流动相。结果:方法测定低限为5.0mg/kg,在5.0～500.0mg/kg添加水平范围内,芝麻酚回收率为81.5%～90.9%,相对标准偏差为1.8%～3.5%。结论:方法准确度和稳定性较好,操作简单快捷,样品净化效果好,测定低限能够满足限量要求。Objective：A UPLC method for the determination of sesamol in sesame oil had been developed. Methods：Sesamol was extracted from sample with 90% acetonitrile-water,and dilute with water to volume. The analyte was determinated by UPLC for the quantitative determination,and quantified by the external standard curve. The separation was performed on a UPLC BEH C18 column with a system of water - acetonitrile as mobile phase. Results：The limit of determination of this method was 5.0mg/kg. The detection limit of 5.0~ 500.0mg/kg proved to be much better than the previously reported one. Average recoveries of sesamol ranged from 81.5% to 90.9% ,with relative standard deviations between 1.8% ~3.5%. Conclusion.The result demonstrated that the method was simple,accurate and suitable for the identification and quantification of sesamol in sesame oil.

关 键 词：超高效液相色谱 芝麻油 芝麻酚 测定 

分 类 号：TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]