虾夷扇贝下脚料糖胺聚糖的化学组成与生物学功能研究  

Study on chemical composition and biological activities of glycosaminoglycan from Patinopecten yessoensis waste

作　　者：李广靖[1] 崔青曼[1] 袁春营[1] 

机构地区：[1]天津市海洋资源与化学重点实验室,天津科技大学海洋科学与工程学院,天津300457

出　　处：《食品工业科技》2012年第24期134-136,139,共4页Science and Technology of Food Industry

基　　金：天津市科技兴海项目(KX2010-0003)

摘　　要：从虾夷扇贝软体部下脚料中提取纯化糖胺聚糖,进行化学组成与生物学功能研究,结果表明,糖胺聚糖的硫酸根含量为5.75%±0.42%,糖醛酸含量为25.86%±1.35%,氨基糖含量为1.03%±0.26%;糖胺聚糖具有较强的清除羟基自由基和一定的清除超氧阴离子自由基的能力,但作用弱于维生素C;糖胺聚糖对肝癌细胞系HepG2细胞有较强的抑制作用;体外实验证实糖胺聚糖可延长TT、PT、APTT,但与肝素相比,活性明显低弱。The chemical composition and biological activities of pure glycosaminoglycan from Patinopecten yessoensis waste were studied. The result showed that：the content of sulfate,uronic acid and amino sugar in glycosaminoglycan was 5.75% ±0.42% ,25.86% ±1.35% and 1.03% ±0.26% respectively. Glycosaminoglycan had a strong scavenging capacity of hydroxyl radical and a certain scavenging capacity of superoxide anion radical,but the effect was weaker than that of vitamin C. Glycosaminoglycan had strong inhibition on HepG2 celI,Glycosaminoglycan could extended the time of TI-, PT and APTT in vitro experiment, but its activities were lower than that of heparin.

关 键 词：虾夷扇贝下脚料 糖胺聚糖 化学组成 生物学功能 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]