响应面优化微波法提取山菠菜多糖的工艺及不同采摘期多糖含量的测定  

Optimizing microwave extraction process of polysaccharide from french spinach by response surface and determinating the contents of polysaccharide of different picking periods

作　　者：沈潘潘[1] 常丽新[1] 张会宜[1] 王华磊[1] 贾长虹[1] 

机构地区：[1]河北联合大学生命科学学院,河北唐山063009

出　　处：《食品工业科技》2012年第24期334-338,共5页Science and Technology of Food Industry

基　　金：河北省科技厅科技支撑项目(07220701D-5)

摘　　要：为优化微波法提取山菠菜多糖的工艺参数,在单因素实验基础上,选择粒度、液料比、微波时间、pH进行四因素三水平的中心组合设计实验,利用Design-Expert软件进行响应面分析,得出微波法提取山菠菜多糖的最佳工艺条件为:粒度100目、液料比28∶1(mL/g)、微波时间4.5min、pH为4.6,多糖提取率实测值为3.25%,预测值为3.27%,实测值与预测值相差甚小,相对误差为1.23%,可确定利用响应面优化微波法提取多糖的工艺参数可靠,有一定的应用价值。在最佳提取工艺条件下,对不同采摘期的山菠菜多糖进行了提取测定,结果表明,不同采摘期多糖提取率差异较小。In order to optimize the process parameters of the microwave extraction of polysaccharide from french spinach,four factors：size,solvent/material ratio,extraction time and pH were optimized. Response surface method optimization of four factors and three levels was processed by the Design-Expert response surface based on single factor tests. Obtained the optimal parameters of the microwave extraction.sizel00,solvent/material 28：1, extraction time 4.5min and pH4.6,the yield of polysaccharides was 3.25% ,compared to the theoretical value （3.27%）,the relative error was 1.23%. The optimization of the polysaccharide extract was reliable and have a certain using value,and in the base of optimal parameters,different picking periods polysaccharide was extracted and determinated,the results showed that the contents of polysaccharide of different picking periods was smaller.

关 键 词：山菠菜 多糖 提取 响应面 

分 类 号：Q949.91[生物学—植物学]