非脱羧勒克菌YT-1107生理生化特性研究及16S rRNA序列分析  

Physiological and biochemical characteristics of Leclercia adecarboxylata YT-1107 and 16S rRNA sequencing

作　　者：伍海燕[1] 李振军[2] 宋燕[1] 张萍[1] 孙振璐[1] 姜照[1] 金东[2] 韩文清[1] 

机构地区：[1]烟台市疾病预防控制中心,山东烟台264003 [2]中国疾病预防控制中心,北京100021

出　　处：《中国食品卫生杂志》2012年第6期536-538,共3页Chinese Journal of Food Hygiene

摘　　要：目的菌株YT-1107是一株从污染食物中分离得到的非脱羧勒克菌,对其进行生理生化特性研究及16S rRNA序列分析确定其归属。方法根据流行病学调查及临床表现,选择疑似病原菌,用ATB微生物自动鉴定系统对采集的样品进行病原菌分离与鉴定。对菌株的16S rRNA基因测序结果进行同源性分析,采用MEGA4.0软件的Neighbor-Joining方法构建系统发育树。结果对该菌株生理生化特征的研究及16S rRNA序列分析表明,该菌株是革兰阴性菌,属于Enterobacter asburiae。结论通过16S rRNA基因序列的同源性比对,系统进化树的构建,初步认定菌株YT-1107与Enterobacter asburiae LF7a为同一物种。Objective YT-1107 is a Leclercia adecarboxylata strain isolated from contaminated food.The study of physiological and biochemical characteristics and 16S rRNA sequencing was aimed to identify its species.Methods Suspected pathogens were selected according to epidemiological survey and clinical manifestations,and then samples were isolated and identified by ATB Biolog microbial identification system.16S rRNA gene sequencing results were analyzed for homology and MEGA4.0 Neighbor-Joining method was used to construct phylogenetic tree.Results Based on the physiological and biochemical characteristics and 16S rRNA sequencing,the strains were gram negative bacteria,belonging to Enterobacter asburiae.Considering the evidence of homology comparison of 16S rRNA gene sequencing and phylogenetic tree construction,this strain was preliminary identified as the same species as Enterobacter asburiae LF7a.Conclusion Strain YT-1107 and Enterobacter asburiae LF7a are the same species.

关 键 词：非脱羧勒克菌 鉴定 16S RRNA 序列分析 食品安全 

分 类 号：TS207.4[轻工技术与工程—食品科学] R155.5[轻工技术与工程—食品科学与工程]