Tween 80与Span 80在食品添加剂微生物学检验中的应用  

Application of Tween 80 with Span 80 in Dilution Systems Used for Oily Food Additives Microbial Examination

作　　者：田金秋[1] 张萍[1,2] 郑大威[1,2] 王晓萌[1] 钟儒刚[1,2] 

机构地区：[1]北京工业大学生命科学与生物工程学院,北京100124 [2]北京市饮料及食品添加剂质量监督检验站,北京100124

出　　处：《中国农学通报》2012年第33期307-310,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"环境污染物暴露与病毒因素协同致癌作用的人胚肝细胞恶性转化检测模型的构建与应用"(21107005);教育部博士点基金项目"环境污染物与病毒因素协同致癌作用的人类细胞检测平台的构建"(20111103120011);北京工业大学博士科研启动基金"利用人类永生化细胞构建环境污染物致癌风险评价体系及其机理研究"

摘　　要：根据国标GB4789规定方法对富含油脂类食品进行微生物学检验时,由于样品梯度稀释困难,往往会造成取样量不准确,对检验结果造成不良影响。为此将表面活性剂按照一定的复配比例应用于富含油脂类食品的梯度稀释过程中,并评价样品乳化效果及其对微生物生长的影响。研究结果表明,v(Tween 80):v(Span 80)=1:1,添加浓度为1%～5%时,对微生物生长没有促进与抑制作用,在对富含油脂类产品(含油量在95%以上)进行梯度稀释时,添加浓度为2%即可达到较好的乳化效果。该方法建立了更为完善的样品前处理方式,能够方便、准确地取样,对于改善富含油脂类食品的微生物学检验具有一定的参考价值。The oily food additives are difficult to be exactly diluted using sterilized physiological saline or phosphate buffer solution according to GB 4789 methods to detect the microbial contaminations. And the inappropriately diluted manner affects the result accuracy directly. This research had established a proper dilution method through application of compound suffaetants in the pretreatment processions. The emulsifying powers and microbial growth effects of Tween-80 compounded with Span-80 had been studied. The results showed that added compound suffactants of Tween-80 with Span-80 （1：1） in the concentrations from 1% to 5% had no effects on microbial growth. And the oily food additives （oil content 〉95%） had been relatively stable emulsificated by only 2% of compound suffactants in the dilution system. This research provided convenient and accurate sample pretreatment techniques which were especially useful for oily foods to detect the microbial contaminations.

关 键 词：表面活性剂 乳化作用 前处理 食品添加剂 

分 类 号：R155[医药卫生—营养与食品卫生学]