酵母菌乳杆菌共培养产谷胱甘肽条件的研究  

Research on Condition of Co-Culture Glutathione by Yeast and Lactobacillus

作　　者：尤晓清[1] 许激扬[1] 卞筱泓[1] 赵玉成[1] 向禧[1] 赵刚刚[1] 邵飞[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2012年第6期512-516,共5页Pharmaceutical Biotechnology

摘　　要：通过酿酒酵母和干酪乳杆菌共培养,在单因素实验的基础上,考察Plackett-Burman实验、正交实验,优化酵母菌和乳杆菌共培养的条件,以提高酵母菌产谷胱甘肽的产量。首先进行酵母菌和乳杆菌共培养的单因素实验,挑选出乳糖浓度、发酵培养基初始pH值、共培养温度、转速、乳杆菌接种量、接种时间、发酵时间,这7个对谷胱甘肽产量有影响的因素。利用分析软件Minitab16,对这7个因素进行Plackett-Burman实验,筛选出3个影响较为显著的因素。利用分析软件SPSS 19,再对这3个因素进行正交实验,得出最佳发酵条件。在最佳发酵条件下,谷胱甘肽产量比优化前提高了35.14%;相同条件下,比单菌培养提高了33.35%。得到最佳发酵条件为:乳糖浓度40 g/L,发酵培养基初始pH值为6.4,共培养温度为36℃,转速为200 r/min,乳杆菌接种量为8%,乳杆菌接种时间为8 h,发酵时间为24 h。酵母菌乳杆菌共培养产谷胱甘肽的方法具有可行性,并且利用Plackett-Burman设计和正交设计可有效优化发酵条件。Optimized co-culture conditions were obtained by Plackett-Burman design and orthogonal design with the co-culture of Saccharomyces cerevisiae and Lactobacillus casei based on single factor experiment. It was to promote the yield of glutathione produced by Saccharomyces cerevisiae. Alloxan method was used to measure the yield of glutathione. Single factor experiment, Plackett-Burman design and orthogonal design were applied to optimize the co-culture condition. First, the single factor experiment of co-culture was to select the factors, lactose concentration, initial pH value of fermentation media, co-culture temperature, co-cuhure rotation speed, inoculum amount of Lactobacillus, inoculum time of Lactobacillus and fermentation time, relevant to the yield of glutathione. On the basis of the results of single factor experiment, the two levels of the seven factors for Plackett-Burman design was identified. Minitab 16 is a statistical software which was applied to conduct the Plackett-Burman design of the seven factors. Three significant impact factors were screened based on the value of P. And positive or negative effects of all factors were determined. The orthogonal experi- ment of the three factors was carried by SPSS 19 to achieve the optimized fermentation condition. The optimized fermentation condi- tions were obtained according to the best mean value corresponding to every factor of glutathione yield. The optimized fermentation conditions were as follows ： lactose concentration 40 g/L, initial pH value of fermentation media 6.4, co-culture temperature 36 ℃, co-cuhure rotation speed 200 r/min,inoculum amount of Lactobacillus 8%, inoculum time of LactobaciUus 8 h and fermentation time 24 h, The yield of glutathione was 1.93mmol/L and increased by 35.14% under the optimized fermentation condition compared with the previous condition. It was increased by 33.35% under the same fermentation condition compared with monoculture. It was feasible to produce glutathione by co-culture of Saccharomyces cerevisiae and

关 键 词：酵母菌 乳杆菌 共培养 谷胱甘肽 优化 

分 类 号：TQ92[轻工技术与工程—发酵工程]