高效液相色谱法同时测定食品中8种合成着色剂  

Simultaneous determination of eight kinds of synthetic colorants in food by high performance liquid chromatography

作　　者：杨岚[1] 张敬轩[1] 李强[1] 吴春敏[1] 李建文[1] 

机构地区：[1]河北省食品质量监督检验研究院,河北省食品安全重点实验室,河北石家庄050091

出　　处：《河北省科学院学报》2012年第3期49-53,共5页Journal of The Hebei Academy of Sciences

基　　金：河北省质量技术监督局科技项目(090107);河北省科技支撑计划项目(12396907D)

摘　　要：建立了高效液相色谱法同时测定食品中8种合成着色剂(柠檬黄、苋菜红、胭脂红、日落黄、诱惑红、亮蓝、酸性橙、赤藓红)的方法。样品经超声提取、离心定容、过膜,经Warters-C18(250mm×4.6mm,5μm)色谱柱分离,以甲醇+0.01mol/L乙酸铵为流动相进行梯度洗脱,用二极管阵列检测器,在254nm、629nm波长处检测,8种物质的线性相关系数大于0.998,方法检出限为0.0502～0.6119μg/mL。8种合成着色剂的回收率范围在85.4%～99.3%之间,RSD(n=6)范围在1.36%～3.89%之间。方法简便、准确、快速。An analytical method to simultaneously determine 8 synthetic colorants in food such as citri amaranth,cochineal, sunset yellow, allura red, using high performance liquid chromatography. volume and filtration, the methano 1--0.01mol/L a length 254 nm,629nm on limits of dete 99.3 % with samples were mmonlum ace a diode array ction of the method were f brilliant blue, acid orange and After ultrasonic extraction, ce erythrosine ntrifugal sep was establis ne, hed aratlon, constant separated on a Warters-C18 column （250mm×4.6mm,5μm） using tate as the mobile phase and detected by gradient elution at wave- detector. All the correlation coefficient is larger than 0. 998. The rom 0. 0502 to 0. 6119μg/mL. The recovery is between 85.4 % and RSD（n=6） of 1.36%-3.89 %.

关 键 词：高效液相色谱法 合成着色剂 食品 检测 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS264.21[轻工技术与工程—食品科学与工程]