高效液相色谱法同时测定植物油中4种甾醇烯  

Simultaneous Determination of Four Steradienes in Vegetable Oils by High Performance Liquid Chromatography

作　　者：于瑞祥[1] 杨瑞钰 张欣[1] 方晓明[3] 丁卓平[1] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海市标准化研究院,上海200031 [3]上海出入境检验检疫局,上海200135

出　　处：《分析化学》2012年第12期1902-1906,共5页Chinese Journal of Analytical Chemistry

基　　金：国家质检总局科研项目(No.2012IK184);上海市教育委员会重点学科建设项目(No.J50704)资助

摘　　要：建立了高效液相色谱法同时测定植物油中4种甾醇烯的方法。样品用石油醚提取,硅胶柱净化,反相C30柱(250 mm×4.6 mm i.d,5μm)分离,乙腈-叔丁基甲醚(75∶25,V/V)作流动相,等度洗脱,紫外检测(235 nm)。结果表明:4种甾醇烯的质量浓度在0.025～1.0 mg/L范围内;峰面积与质量浓度呈良好的线性关系,线性相关系数r2>0.999。在加标水平为0.050,0.10和0.50 mg/kg时,4种甾醇烯的加标平均回收率为89.2%～109.8%;相对标准偏差RSD<8%;方法检出限(LOD)为0.010 mg/kg。本方法已应用于实际样品分析。A method for the simultaneous determination of four steradienes in vegetable oils by high performance liquid chromatography（HPLC） was established.The sample was dissolved in petroleum ether and passed through a silica gel column,and the analytes were eluted with petroleum ether.The chromatographic separation was carried out on a C30 column（250 mm×4.6 mm i.d,5 μm） using UV detection at 235 nm.Acetonitrile/tert-butyl methyl ether（75 ∶ 25,V/V） was served as mobile phase in isocratic elution.Results showed good linear ranges for 4 steradienes from 0.025 mg/L to 1.0 mg/L with correlation coefficients（r2）0.999.The mean recoveries were in the range of 89.2%-109.8% at three spiked levels of 0.050,0.10 and 0.50 mg/kg,and the relative standard deviations（RSDs） were less than 8 %.The limits of detection（LODs） were of 0.010 mg/kg.The proposed method had been applied in real sample analysis.

关 键 词：高效液相色谱 甾醇烯 植物油 

分 类 号：TS227[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.72[轻工技术与工程—食品科学与工程]