胃癌有丝分裂关卡基因BUB1和BUBR1 mRNA的表达  

Expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA in gastric cancer

作　　者：余晓峰[1] 冯全林[1] 朱震[1] 陈海军[1] 王雪林[1] 徐正平[1] 钱宏亮[1] 吴江[1] 吴清[1] 陆琦[1] 

机构地区：[1]昆山市中医医院肿瘤外科,江苏省215300

出　　处：《江苏医药》2012年第24期2978-2980,共3页Jiangsu Medical Journal

摘　　要：目的研究胃癌组织中有丝分裂关卡基因BUB1和BUBR1的表达。方法采用半定量RT-PCR技术检测39例胃癌组织及正常胃组织中BUB1及BUBR1基因的表达。结果胃癌组织中BUB1mRNA/β-actin mRNA和BUBR1mRNA/β-actin mRNA拷贝数比值均明显高于正常胃组织(1.84±0.43 vs. 0.86±0.44和2.00±1.36 vs. 0.96±0.60)(P<0.05)。结论 BUB1基因和BUBR1基因在胃癌中高表达可能与癌细胞的异常增殖有关。Objective To study the expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA in gastric cancer. Methods Using semi-quantitative PCR, the expressions of mitotic checkpoint gene BUB1 and BUBR1 mRNA were detected in 39 gastric cancer samples（group A） and the tumor-adjacent normal tissues（group B）. Results The ratios of BUB1/β-actin gene rnRNA copies and BUBR1/β-actin gene mRNA copies in group A were significantly higher than those in group B （1.84±0.43 vs. 0.86±0.44 and 2.00±1.36 vs. 0.96±0.60）（P〈0.05）.Conclusion BUB1 and BUBR1 genes are highly expressed in gastric cancer, which may be associated with abnormal proliferation of cancer cells.

关 键 词：胃癌 有丝分裂关卡基因 BUB1 BUBR1 

分 类 号：R735[医药卫生—肿瘤]