Peroxynitrite Scavenging Activities of Resveratrol and Piceid  

作　　者：ZHAO Guang-rong TIAN Li-li MA Qiong WANG Chang-song QIAO Bin ZHANG Jun-gang JI Xiang-wu 

机构地区：[1]Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemieal Engineering and Teehnology, Tianjin University, Tianjin 300072, P. R. China [2]Department of Cardiology, Weifang People's Hospital, Weifang 261041, P. R. China

出　　处：《Chemical Research in Chinese Universities》2012年第6期953-956,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Basic Research Program of China(Nos.2012CB721105,2011CBA00802,2007CB714301);the National Natural Science Foundation of China(No.30873400);the Specialized Research Fund for the Doctoral Program of Higher Education of China(No.20090032110015)

摘　　要：In vitro antioxidant activities of resveratrol and piceid against peroxynitrite（ONOO-） were examined by the inhibition of 3-nitrotyrosine formation.Trolox was used as a positive control.Resveratrol and piceid exhibited high ONOO--scavenging activities in a concentration dependent manner.The antioxidant activities（the concentration of test compound required to yield a 50% inhibition of tyrosine nitration,IC 50） of resveratrol and piceid against ONOO-were（48.34±0.97） and（74.69±1.49） μmol/L,respectively.Compared with that of trolox[（105.40±1.16） μmol/L],their scavenging activities were 2.2-and 1.5-fold higher for resveratrol and piceid.Formation of nitroresveratrol as shown by UV-Vis spectroscopy and liquid chromatography-tandom mass spectrometry（LC-MS/MS） analysis indicates that resveratrol could directly scavenge ONOO-via nitration reaction.Our results demonstrate that foods and medicinal herbs with resveratrol and piceid as stronger ONOO-scavengers are valuable ingredients and have healthy application in preventing humans from peroxynitrite-mediated oxidative damage by scavenging peroxynitrite efficiently.In vitro antioxidant activities of resveratrol and piceid against peroxynitrite（ONOO-） were examined by the inhibition of 3-nitrotyrosine formation.Trolox was used as a positive control.Resveratrol and piceid exhibited high ONOO--scavenging activities in a concentration dependent manner.The antioxidant activities（the concentration of test compound required to yield a 50% inhibition of tyrosine nitration,IC 50） of resveratrol and piceid against ONOO-were（48.34±0.97） and（74.69±1.49） μmol/L,respectively.Compared with that of trolox[（105.40±1.16） μmol/L],their scavenging activities were 2.2-and 1.5-fold higher for resveratrol and piceid.Formation of nitroresveratrol as shown by UV-Vis spectroscopy and liquid chromatography-tandom mass spectrometry（LC-MS/MS） analysis indicates that resveratrol could directly scavenge ONOO-via nitration reaction.Our results demonstrate that foods and medicinal herbs with resveratrol and piceid as stronger ONOO-scavengers are valuable ingredients and have healthy application in preventing humans from peroxynitrite-mediated oxidative damage by scavenging peroxynitrite efficiently.

关 键 词：RESVERATROL PICEID PEROXYNITRITE 3-NITROTYROSINE Antioxidant activity Nitration reaction Radical scavenging 

分 类 号：O658[理学—分析化学] Q463[理学—化学]