不同窖龄窖泥古菌的系统发育多样性分析  

Phylogenetic Diversity Analysis of Archaeal in the Pit Mud with Different Cellar Age

作　　者：王明跃[1,2] 张文学[2] 王海英[2] 刘超兰[2] 

机构地区：[1]阜阳职业技术学院,阜阳236031 [2]四川大学轻纺与食品学院,成都610065

出　　处：《应用与环境生物学报》2012年第6期1043-1048,共6页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金面上项目(No.31071585);安徽省高校省级自然科学研究项目(No.KJ2012B140)资助~~

摘　　要：为探索不同窖龄窖泥古菌类群与演替规律，分别提取20、50和150年窖龄窖泥样品总DNA，应用PCR—ARDRA和16SrDNA克隆测序技术对不同窖龄窖泥古菌群落结构进行研究．结果表明：191个克隆子分成22个可操作分类单元（OTUs），系统发育分析归为6个类群，分别属于甲烷八叠球菌属（Methanosarcina）、甲烷杆菌属（Methanobacterium）、甲烷袋状菌属（Methanoculleus）、甲烷粒菌属（Methanocorpuseulum）、甲烷鬃菌属（Methanosaeta）和甲烷螺菌属（Methanospirillum）．不同窖龄窖泥的优势古菌类群有明显差异，20年窖泥优势古茵类群以乙酸利用型Methanosarcina、Methanosaeta为主，50年、150年龄窖泥优势古菌类群以氢气营养型Methanocorpusculum、Methanobacterium为主；随着窖龄的增加，窖泥古菌Shannon．Wiener多样性指数（日）和Berger-Parker优势度指数（D）呈增加的趋势，Pielou均匀度指数（，）呈降低趋势；不同窖龄窖池古菌的相似性系数为0．429～0．677，50年和150年窖泥的菌群结构最为相似．本研究揭示了不同窖龄窖泥古菌系统发育多样性的差异与窖泥老熟的关系．In order to investigate the clusters and succession rules of archaeal in the pit mud, the total DNAs were extracted from the pit mud with cellar age of 20, 50 and 150 years, respectively. The community structure was studied by polymerase chain reaction-amplified rDNA restriction analysis （PCR-ARDRA） and sequencing of 16S rDNA. The results showed that 191 clones from the 16S rDNA library fell into 22 operational taxonomic units （OTUs） from 6 groups including Methanosarcina, Methanobacterium, Methanoculleus, Methanocorpusculum, Methanosaeta and Methanospirillum. Diversity was obvious in the predominant archaeal from the pit mud with different cellar age. The predominant archaeal from the pit mud with cellar age of 20 years were Methanosarcina and Methanosaeta, which produced methane by utilizing acetic acid. However, the predominant archaeal from the pit mud with cellar age of 50 and 150 years were Methanocorpusculum and Methanobacterium, which utilizing hydrogen. With the increasing cellar age, the Shannon-Wiener index （H） and Berger-Parker index （D） of archaeal in the pit mud showed an increasing trend, while the Pielou index （J） showed a decreasing trend. The Sorenson similarity index of the pit mud with different cellar age was the range of 0.429~0.677, and there was the highest similarity of community structure between the pit mud with cellar age of 50 and 150 years. This study revealed the relationship between archaeal phylogenetic diversity of pit mud and the aging of pit mud.

关 键 词：窖泥 古菌类群 16S RDNA序列 克隆文库 系统发育 多样性 

分 类 号：TS261.1[轻工技术与工程—发酵工程]