UPLC-MS/MS法测定生咖啡中的赭曲霉毒素A  

Detection of Ochratoxin A in Green Coffee by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

作　　者：乐渊[1] 刘春华[1] 徐志[1] 万瑶[1] 苟亚峰[2] 

机构地区：[1]中国热带农业科学院分析测试中心,海南海口571101 [2]中国热带农业科学院香料饮料研究所,海南万宁571533

出　　处：《热带农业科学》2012年第12期87-90,共4页Chinese Journal of Tropical Agriculture

基　　金：公益性行业(农业)科研专项(No.200903024-03)

摘　　要：建立了生咖啡中赭曲霉毒素A的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品用甲醇提取后,直接在BEH C18色谱柱上以乙腈-0.1%甲酸水为流动相分离,采用多离子监测模式(MRM)进行测定。赭曲霉毒素A的线性范围为5.0～200.0 ng/mL,相关系数大于0.99,检出限为0.5μg/kg。4个水平的添加回收率在84.3%～109.2%,相对标准偏差小于7.35%。本方法灵敏度高、准确性好、成本低,适用于生咖啡中赭曲霉毒素A的检测。An analytical method was developed for the determination of ochratoxin A in green coffee by using ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS）. OTA was extracted by methanol, separated through BEH C18 column using acetonitrile/water （containing 0.1% formic acid） as mobile phase, and then quantified with multiple reactions monitoring （MRM）. Calibration curves exhibited good linearity over the concentration range from 5.0 to 200.0 ng/mL, with the correlation coefficients above 0.99. The limit of detection （LOD） for OTA was 0.5 izg/kg. Average recovery for OTA spiked at 4 levels ranged from 84.3% to 109.2%, with a relative standard deviations （RSD） less than 7.35%. The developed method was sensitive, accurate, low-cost, and can be applied for determination of OTA in green coffee.

关 键 词：赭曲霉毒素A 超高效液相色谱-串联质谱法 生咖啡 真菌毒素 

分 类 号：S571.1[农业科学—茶叶生产加工]