一株降解玉米秸秆菌株的分离、鉴定及其降解能力的研究  

Study on isolation,identification and degradation capability of a bacteria of degrading corn straw

作　　者：周沅洁[1,2] 马桂英[1,2] 王修俊[1,2] 艾静汶[1] 朱隆绘[1] 

机构地区：[1]贵州大学化学与化工学院,贵州贵阳550025 [2]贵州省发酵工程与生物制药重点实验室,贵州贵阳550025

出　　处：《广东农业科学》2012年第20期87-90,共4页Guangdong Agricultural Sciences

基　　金：贵州省科技厅社会发展科技攻关项目〔黔科合SY(2010)3041〕;贵州省工业攻关项目〔黔科合GY(2008)3018〕

摘　　要：从玉米秸秆上分离筛选出的S-3菌株能够分泌纤维素酶,具有降解农作物秸秆的能力。该菌经鉴定为芽孢杆菌属,其产纤维素酶活为172.786 U/g,最佳降解条件为发酵时间11 d、发酵温度32℃、料水比1∶2.5、S-3菌株用量10 mL。在上述条件下,纤维素占发酵干基的比率从最初的35.48%降到23.26%;木质素从最初的15.37%降到14.51%。In this study,we isolated strains from the corn straw which could secrete cellulose and degraded straw.This bacteria was identified as Bacillus,and its cellulase activity was 172.786 U/g.The best degradation conditions were as follows： fermentation time of 11 days,culture temperature 32℃,culture medium material ratio 1∶2.5,inoculation of strains 10 mL.Under these conditions,the proportion of cellulose dropped from 35.48% to 23.26%,and the lignin dropped from 15.37% to 14.51%.

关 键 词：秸秆 分离筛选 鉴定 降解能力 

分 类 号：S182[农业科学—农业基础科学]