黑灵芝多糖对阿霉素大鼠心肌损伤的保护作用  

Protective Effect of Polysaccharides from Ganoderma atrum against Adriamycin-Induced Myocardial Injury in Rats

作　　者：李文娟[1] 聂少平[1] 邵灯寅[1] 朱科学[1] 李娜[1] 郑温雅[1] 谢明勇[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《食品科学》2012年第23期283-286,共4页Food Science

基　　金：国家自然科学基金项目(31071532;30960242;31201326);“十二五”国家科技支撑计划项目(2012BAD33B06);食品科学与技术国家重点实验室目标导向项目(SKLF-MB-201001)

摘　　要：目的:探讨黑灵芝多糖对阿霉素(ADR)大鼠心肌损伤的保护作用。方法:腹腔注射ADR,建立心肌损伤模型,随机分为正常对照组、黑灵芝多糖高中低剂量组、ADR模型组,观察大鼠生理状况,检测乳酸脱氢酶(LDH)、肌酸激酶(CK)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性及线粒体膜电位、丙二醛(MDA)的含量。结果:与ADR模型组相比,黑灵芝多糖显著改善ADR引起体质量下降,提高CAT、GSH-Px、SOD活性和线粒体膜电位,降低LDH、CK活性和MDA含量。结论:黑灵芝多糖对ADR心肌损伤具有保护作用,其作用机制与降低心肌氧化应激、提高抗氧化防御、保护线粒体有关。Objective： To investigate the protective effect of Ganoderma atrum polysaccharides against adriamycin（ADR）induced cardiotoxicity in SD rats.Methods： A myocardial injury model induced by intraperitoneal injection of ADR was established in SD rats.SD rats were divided arbitrarily into five groups： control group,ADR model group,and three Ganoderma atrum polysaccharide treatment groups（administered at doses of 25,50 mg/（kg.d） and100 mg/（kg.d） body weight,respectively for 7 consecutive days）.ADR was administered on the 8th day at a dose of 20 mg/kg body weight.On the 14th day,the mice in each group were sacrificed and hearts were collected and determined for the activities of lactate dehydrogenase（LDH）,creatine kinase（CK）,catalase（CAT）,glutathione peroxidase（GSH-Px） and superoxide dismutase（SOD）,mitochondrial membrane potential and malondialdehyde（MDA） content.Moreover,mouse body weight and physiological conditions were examined.Results： Compared with ADR model group,Ganoderma atrum polysaccharides significantly attenuated ADR-induced body weight loss,increased CAT,SOD and GSH-Px activities and mitochondrial membrane potential,and decreased LDH and CK activities and MDA content in cardiac tissues.Conclusions： Ganoderma atrum polysaccharides can protect against ADR-induced myocardial injury in rats.The mechanism may be related to reducing oxidative stress,enhancing antioxidant defense and protecting mitochondria.

关 键 词：黑灵芝多糖 阿霉素 心肌损伤 氧化应激 

分 类 号：Q949.32[生物学—植物学]