满江红鱼腥藻glnA启动区的克隆及在烟草中的活性表达  

THE CLONE OF ANABAENA AZOLLAE GLNA PROMOTER AND ACTIVITY EXPRESSION IN TRANSGENIC TOBACCO

作　　者：刘祥林[1] 印莉萍[1] 吴燕川 高志环[1] 吴晓强[1] 

机构地区：[1]首都师范大学生物系,北京100037 [2]宣武医院多肽室,北京100053

出　　处：《水生生物学报》2000年第4期369-373,共5页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金!(39270414)

摘　　要：利用ＰＣＲ技术获得满江红鱼腥藻ｇｌｎＡ启动区，经克隆测序后构成谷氨酰胺合成酶基因启动于驱动的ＧＵＳ基因表达载体。用基因枪转化，将表达载体导入烟草中，在其叶片和茎中检测到ＧＵＳ活性，而在烟草根中未见表达。实验结果对真核生物与原核生物间基因表达调控、转录因子识别的研究以及构建实用载体具有一定价值。The promoter of Anabaena aazollae glnA gene was obtained by PCR procedure. A binary vector containing the chimeric gene of glnA / GUS was constructed after cloning and sequencing. The expression vector was transformed into tobacco by microprojectile bombardment. 'The GUS activities were examined histochemically in leaves and stems of the transgenic tobacco, but not in its roots. The result was valuable both in the regulation of gene expression, the recognition of transcriptional factors between eukaryota and prokaryota and the construction of practical vectors.

关 键 词：glnA启动区 满江红鱼腥藻 烟草 基因表达 PCR 

分 类 号：Q75[生物学—分子生物学] Q949.221