食品过敏原大豆P34蛋白基因的实时荧光PCR检测  

Detection allergen soybean in food using real-time PCR

作　　者：张舒亚[1] 于翠[1] 宋青[1] 于卓然[1] 高琴[1] 

机构地区：[1]上海出入境检验检疫局,上海200135

出　　处：《中国油料作物学报》2012年第6期661-665,共5页Chinese Journal of Oil Crop Sciences

基　　金：上海技术标准专项(08DZ0505200;10DZ0504300);国家质检公益性行业科研专项(10-46)

摘　　要：以大豆主要过敏蛋白P34基因为靶标,采用TaqMan探针实时荧光PCR方法建立食品过敏原大豆成分的检测方法。实验表明建立的检测方法具有特异性,灵敏度高,检测限为10mg/kg。通过对市售样品的检测,该方法适用于对常见食品种类的检测,但不适用于精加工油脂等精细加工产品的检测。A real-time PCR method was established for detecting soybean DNA in food by using primers and probe corresponding to soybean immunodominant allergen P34.The method was specific for soybean and did not show any cross-reactivity with common food ingredients.The limit of detection was determined as 10mg/Kg.The PCR procedure is suitable for detecting soybean in food except refined products.

关 键 词：食品过敏原 大豆 实时荧光PCR方法 检测 

分 类 号：Q754[生物学—分子生物学] S565.1[农业科学—作物学]