无花果ACC合成酶基因克隆及序列分析  

Cloning and Sequence Analysis of a DNA Encoding ACC Synthase Gene from Ficus carica

作　　者：张帅[1] 张明[1] 寇天舒[1] 马俊莲[1] 唐霞[1] 张子德[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071001

出　　处：《华北农学报》2012年第6期34-37,共4页Acta Agriculturae Boreali-Sinica

摘　　要：为进一步利用基因工程手段调控无花果乙烯的合成,以无花果果肉为材料提取基因组DNA,根据已报道的无花果ACC合成酶基因序列设计引物,采用PCR技术扩增得到一条约600 bp的特异片段,将该片段克隆到pGM-Teasy vector上经PCR、酶切和测序鉴定。序列分析结果表明,基因全长590 bp,编码196个氨基酸,该序列与GenBank上已登陆的Masui Dauphine-ACS1的cDNA序列同源性达99%,氨基酸同源性达98%。结果表明,成功克隆到了无花果ACS基因片段。Specific primers were designed based on the the reported ACC synthase（ACS）gene sequences of Ficus carica in the GenBank.Using genome DNA extracted from Ficus carica fruits as template the specific PCR product of ACS was obtained,then the product was ligased to pGM-T easy vector with PCR,restriction enzyme digestion and sequencing.The sequencing data showed that the PCR product was 590 bp encoding 196 predicted amino acid residues.Comparison with nucleotide sequence and amino acid sequence homology of ACC oxidase gene（DQ269492）landed were 99% and 98%,which showed the successful cloning of the ACSI synthase gene.

关 键 词：无花果 ACC合成酶 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学]