牛血清蛋白分子印迹聚合物的制备及分子识别性能  

Fabrication of Bovine Serum Album in Imprinted Polymer on Silica Surface and Its Molecular Recognition Function

作　　者：袁士芳[1] 王硕[1] 邓启良[1] 方国臻[1] 

机构地区：[1]天津科技大学食品营养与安全实验室,天津300457

出　　处：《现代食品科技》2013年第1期134-137,126,共5页Modern Food Science and Technology

摘　　要：本文报道了以活化硅球为支撑载体,将其表面用3-氨基丙基三乙氧基硅烷(APTES)和戊二醛进行修饰,通过醛基共价健和牛血清蛋白(BSA),同时以丙烯酰胺(AAm)为单体,N,N’-亚甲基双丙烯酰胺(MBA)为交联剂,在水相体系中合成对BSA具有较高选择性的新型分子印迹聚合物。通过对合成的聚合物进行平衡结合,吸附动力学和选择性的三个表征实验发现,该聚合物对BSA具有选择性高、传质速度和结合平衡速度快等诸多优点,并通过Langmuir方程分析,证明平衡结合中吸附容量(Q)与初始浓度(C)的一致性,并呈良好的线性关系,印迹聚合物的饱和吸附容量Qmax为28.98 mg/g。A novel molecularly imprinted polymer has been prepared using silica gel as support material, bovine serum albumin （BSA） as template, acrylamide （AAm） as functional monomer and N,N＇-methylenebisacrylamide （MBA） as cross-linker in aqueous medium. The silica gel was modified by 3-aminopropyltriethoxysilane （APTES） and glutaraldehyde and then reacted with BSA by forming imine bonds to covalently immobilize the template protein. The static and kinetic adsorption properties of the imprinted polymer were evaluated. The results showed that the imprinted sorbent exhibited good recognition, selective ability and offered a faster kinetics for the adsorption of the template BSA. The results of the Langmuir model evahtation indicated that the adsorption isotherm of imprinted polymer towards the template molecule were in good accordance and with linearity. The maximum adsorption capacity （Qmax） of imprinted sorbent toward BSA was 28.98 mg/g.

关 键 词：分子印迹 共价健和 牛血清蛋白 活化硅球 

分 类 号：TS201.2[轻工技术与工程—食品科学]