Neural cell injury microenvironment induces neural differentiation of human umbilical cord mesenchymal stem cells  

作　　者：Jin Zhou Guoping Tia Jinge Wang Xiaoguang Luo Siyang Zhang Jianping Li Li Li Bing Xu FengZhu Xia Wang Chunhong Jia Weijin Zhao Danyang Zhao Aihua Xu 

机构地区：[1]Department of Neurology, First People's Hospital of Shenyang, Shenyang 110041, Liaoning Province, China [2]First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China [3]College of Basic Medical Sciences, China Medical University, Shenyang 110001, Liaoning Province, China [4]Liaoning Provincial Blood Center, Shenyang 110044, Liaoning Province, China

出　　处：《Neural Regeneration Research》2012年第34期2689-2697,共9页中国神经再生研究（英文版）

摘　　要：This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells （hUCMSCs） under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation, hUCMSCs were co-cultured with normal or AI31.4o-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone （no treatment）. Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural ceils.This study aimed to investigate the neural differentiation of human umbilical cord mesenchymal stem cells （hUCMSCs） under the induction of injured neural cells. After in vitro isolation and culture, passage 5 hUCMSCs were used for experimentation, hUCMSCs were co-cultured with normal or AI31.4o-injured PC12 cells, PC12 cell supernatant or PC12 cell lysate in a Transwell co-culture system. Western blot analysis and flow cytometry results showed that choline acetyltransferase and microtubule-associated protein 2, a specific marker for neural cells, were expressed in hUCMSCs under various culture conditions, and highest expression was observed in the hUCMSCs co-cultured with injured PC12 cells. Choline acetyltransferase and microtubule-associated protein 2 were not expressed in hUCMSCs cultured alone （no treatment）. Cell Counting Kit-8 assay results showed that hUCMSCs under co-culture conditions promoted the proliferation of injured PC12 cells. These findings suggest that the microenvironment during neural tissue injury can effectively induce neural cell differentiation of hUCMSCs. These differentiated hUCMSCs likely accelerate the repair of injured neural ceils.

关 键 词：stem ceil umbilical cord mesenchymal stem cell CO-CULTURE induction DIFFERENTIATION neural cell microtubule-associated protein 2 injured cell TRANSWELL neural regeneration REGENERATION 

分 类 号：Q254[生物学—细胞生物学] Q42