肠杆菌科细菌在热鲜肉上生长预测模型的建立与验证  

Establishment and verification of predictive growth model of Enterobacteriaceae in hot-boned pork

作　　者：翁丽华[1] 徐幸莲[1] 周光宏[1] 江芸[2] 

机构地区：[1]南京农业大学国家肉品质量安全控制工程技术研究中心,江苏南京210095 [2]南京师范大学金陵女子学院,江苏南京210097

出　　处：《南京农业大学学报》2013年第1期125-130,共6页Journal of Nanjing Agricultural University

基　　金：江苏省农业三项工程项目(sx[2010]268);国家公益性行业(农业)科研专项(200903012);国家自然科学基金项目(31071614)

摘　　要：将肠杆菌科细菌接种到宰后1 h的无污染的热鲜肉上,置于灭菌培养皿上并分别于5、10、15、25和30℃贮藏,建立肠杆菌科细菌在热鲜肉上的生长预测模型,并对模型进行验证。采用SPSS 17.0统计软件拟合特定温度下的生长情况。结果表明:修正的Gompertz模型能很好地描述不同温度下肠杆菌科细菌在热鲜肉上的生长状况。对最大比生长速率和延滞时间建立平方根模型,结果呈良好的线性关系,相关系数(R2)分别为0.97和0.98。用贮藏在8和22℃热鲜肉中肠杆菌科细菌的生长试验值验证所建立的模型,并对偏差度、准确度、残差平方和及均方根误差进行计算和分析,结果表明:建立的模型可靠性高,可有效预测5～30℃肠杆菌科细菌在热鲜肉上的生长。The pollution free hot-boned pork was inoculated with Enterobacteriaceae,put on aseptic plate and stored at 5,10,15,25 and 30 ℃ respectively.The dynamic growth model of Enterobacteriaceae was established and verified.SPSS 17.0 software was used to fit the status of growth at different temperatures.The results indicated that the modified Gompertz model could well describe the growth of Enterobacteriaceae in hot-boned pork.The regression of Belehradek model finely described the effect of temperatures on the maximum specific growth rate and the lag phase,and correlation coefficients of determination were 0.97 and 0.98.The validation of the built model was carried out by comparing the actual and predicted growth curves of Enterobacteriaceae in hot-boned pork at 8 ℃ and 22 ℃ respectively.By calculating bias factor,accuracy factor,the sum of squares due to error and root mean square error,we found that the established model had high reliability and could predict the growth of Enterobacteriaceae on hot-boned pork in the range of temperature from 5 ℃ to 30 ℃ effectively.

关 键 词：肠杆菌科细菌 热鲜肉 生长预测模型 

分 类 号：TS201.3[轻工技术与工程—食品科学]