无载体固定化少根根霉产脂肪酶发酵培养基的优化  

Fermentation medium optimization of lipase produced by the self-immobilization of Rhizopus arrhizus

作　　者：冀颐之[1] 赵有玺[1] 龚平[1] 王群群[1] 谭天伟[2] 

机构地区：[1]北京联合大学生物化学工程学院,北京100023 [2]北京化工大学教育部生物炼制工程中心,北京100029

出　　处：《食品工业科技》2013年第4期171-175,共5页Science and Technology of Food Industry

基　　金：国家"863"项目(2002AA514030);国家"十五"攻关项目(2001BA708B02208);国家自然科学基金项目(2107600C);中石化项目(202059);北京市属高等学校人才强教计划资助项目-中青年骨干人才项目(PHR-201108426);北京市委组织部优秀人才培养资助项目(2012D005022000007)

摘　　要：通过单因子和正交实验,对无载体固定化少根根霉N-103产脂肪酶的发酵培养基进行研究,获得摇瓶发酵的最优培养基条件为:花生油5mL/L、麸皮多糖水解液75mL/L、棉籽蛋白15g/L、(NH4)2SO42g/L、MgSO41g/L、K2HPO42.5g/L。在此条件下进行摇瓶验证实验,少根根霉脂肪酶酶活可达100U/mL,为基础培养基配方对照组的1.33倍。The medium of lipase produced by the self-immobilization of Rhizopus arrhizus N-103 was studied by single factor and orthogonal experiments. The optimum medium consisted of-peanut oil 5ml/L,bran polysaccharide hydrolyzate 75mL/L,cottonseed highest lipase activity 100U/mL was achieved much as the control group. protein 15g/L, （NH4）2SO4 2g/L, MgSO4 1g/L, K2HPO4 2.5g/L The n flask culture in the optimum medium,which was 1.33 times as

关 键 词：脂肪酶 少根根霉 无载体固定化 培养基 

分 类 号：TS201.3[轻工技术与工程—食品科学]