中心组合试验设计响应面分析法优化蜂胶总多酚提取工艺  

Optimization of Extraction Process for Total Polyphenols from Propolis by Central Composite Design Coupled with Response Surface Methodology

作　　者：丁仲鹃[1] 毕玉芬[1,2] 戴云[2] 雷雨[2] 

机构地区：[1]昆明医科大学口腔医院,云南昆明650021 [2]云南民族大学化学与生物技术学院,民族药资源化学国家民委-教育部重点实验室,云南昆明650500

出　　处：《食品科学》2013年第2期17-21,共5页Food Science

基　　金：国家自然科学基金面上项目(30471225);"十一五"国家科技支撑计划项目(2006BAD27B09;2006BAD04A12);国家高技术研究发展计划项目(2007AA10Z320)

摘　　要：利用星点设计-响应面分析法(CCD-RSM)优化蜂胶总多酚的超声提取工艺。在单因素试验基础上,选取超声时间、液料比和乙醇体积分数为影响因素,以Folin-Ciocalteus法测定总多酚含量作为响应值进行响应面分析,确定最佳提取条件。结果表明:超声提取时间24.0min、乙醇体积分数77.0%、料液比1:14(g/mL)时,在此条件蜂胶总多酚提取量为3.34mg/100mg。该提取方法简单快速有效,提高了蜂胶中总多酚的提取率。Central composite design coupled with response surface methodology （CCD-RSM） was employed to optimize the ultrasonic-assisted extraction of total polyphenols from propolis. By using one-factor-at-a-time method, we identified ultrasonic treatment time, solid-to-solvent ratio and ethanol concentration as main variables that influence extraction efficiency. The total polyphenol content of propolis extracts was determined by Folin-Ciocalteus method. Response surface analysis showed that the optimum extraction conditions were 24.0 min, 77.0% and 1：14 （g/mL） for ultrasonic treatment time, ethanol concentration and solid-to-solvent ratio, respectively. Under these conditions, the average extraction yield of total polyphenols from propolis was 3.34 mg/100 mg （n = 3）. This extraction method was simple and efficient and could provide a sample preparation approach for the determination of total polyphenols in propolis.

关 键 词：蜂胶 星点设计-响应面分析法 总多酚 含量 测定 

分 类 号：TS202.3[轻工技术与工程—食品科学]