响应面优化闪式提取香蕉多酚氧化酶  

Response Surface Methodology for the Optimization of Extraction Process for Polyphenol Oxidase from Banana(Musa nana L.) Using Herbal Blitzkrieg Extractor

作　　者：曲留柱[1] 金世超[1] 崔洁[1] 王建中[1] 李军[1] 

机构地区：[1]北京林业大学生物科学与技术学院,北京100083

出　　处：《食品科学》2013年第2期136-142,共7页Food Science

摘　　要：利用闪式提取方法,以香蕉多酚氧化酶的比活力为响应值,首先通过单因素试验考察多酚氧化酶提取缓冲液的种类、离子强度、pH值、液料比、提取时间、聚乙烯吡咯烷酮(PVPP)、聚乙二醇辛基苯基醚(Triton X-100)等因素对香蕉多酚氧化酶提取的影响,初步优化提取条件;进而通过Box-Behnken试验设计和响应面分析法综合优化最终提取条件。得出香蕉多酚氧化酶的理论最佳提取条件为:以磷酸-柠檬酸缓冲液、pH5.58、液料比1.57:1、PVPP质量浓度1.13g/100mL为提取液,用闪式提取器提取2.5 min。Response surface methodology was applied to optimize the extraction of polyphenol oxidase （PPO） from banana （Musa nana L.） pulp using a herbal blitzkrieg extractor system. One-factor-at-a-time method was used to investigate the effects of extraction buffer type, ionic strength and pH, solid-to-solvent ratio, extraction time, polyvinylpyrrolidone （PVPP） concentration, and polyethylene glycol octyl phenyl ether （Triton X-100） concentration at six different levels on the PPO specific activity of banana pulp extracts. Phosphoric acid-citric acid buffer was found to be the best solvent for the extraction of PPO. Extraction buffer pH, solid-to-solvent ratio, extraction time, and PVPP concentration were identified as main affecting parameters and optimized by response surface analysis based on a Box-Behnken experimental design were 5.58, 1.57：1, 2.5 min and 1.13 g/100 mL, respectively.

关 键 词：香蕉 多酚氧化酶 闪式提取 响应面 

分 类 号：Q554[生物学—生物化学]