酪氨酸流加方式对重组大肠杆菌发酵生产L-苯丙氨酸的影响  

Influence of feeding L-tyrosine during induction phase on production of L-phenylalanine in fed-batch cultivation by recombinant Escherichia coli

作　　者：王静[1] 周海岩[1] 李江华[1] 王天文[1] 刘龙[1] 堵国成[1,2] 陈坚[1,2] 

机构地区：[1]江南大学生物工程学院,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《工业微生物》2013年第1期24-29,共6页Industrial Microbiology

基　　金：973项目(2007CB714306);973前期研究专项(2010CB535014);国家杰出青年基金项目(20625619);国家自然科学基金重点项目(20836003);中央高校基本科研业务费专项(JUSRP30901)

摘　　要：研究了流加浓度对酪氨酸重组大肠杆菌Escherichia coli BR-165(pAP-B03)发酵生产L-苯丙氨酸的影响。结果表明,诱导后L-酪氨酸流加加速了菌体的生长,提高了生产强度,缩短了发酵周期。在流加浓度为75 mg/h时,最大菌体干重达到了40.13 g/L(对照11.48 g/L),生产周期缩短到30 h(对照48 h),生产强度达到1.409 g/h/L(对照0.876 g/h/L)。但是L-酪氨酸的流加对L-苯丙氨酸的最终产量没有明显的影响,因此可认为流加酪氨酸是减少发酵时间并提高生产强度的有效方法。本研究获得的酪氨酸流加方式对L-苯丙氨酸的工业化生产具有一定的指导意义。The influence of feeding L-tyrosine （L-Tyr） at different rates during induction phase （after 12 h） on the production of L-phenylalanine （L-Phe） by an L-Tyr auxotrophic Escherichia coli BR-165 （pAP-B03） was investigated. Three feeding rates including 25 mg/h, 50 mg/h and 75 mg/h were examined. The results showed that in comparison with the control （ without L-Tyr feeding）, the feeding of concentrated L-Tyr increased cell growth rate and L-Phe productivity. At a feeding rate of 75 mg/h, the dry cell weight （DCW） increased from 11.48 g/L of the control to 40.13g/L, and the fermentation time decreased from 48 h of the control to 30 h, and the L-Phe productivity increased from 0. 876 g/h/L of the control to 1. 409 g/h/L. However, the feeding of L-Tyr had little influence on the final L-Phe titer, which remained the same as the control. It indicated that feeding L-Tyr at a suitable rate during induction phase was an effective approach to reduce fermentation time and to increase L-Phe productivity. The results may be useful for the industrial production of L-Phe.

关 键 词：L-苯丙氨酸 L-酪氨酸流加 生产强度 比生长速率 比产酸速率 

分 类 号：TQ922[轻工技术与工程—发酵工程]