添加稳定剂提高过氧化氢酶的稳定性  

Improvement of catalase stability by adding stabilizer

作　　者：冯卓林[1] 李江华[1] 刘龙[1] 堵国成[1,2] 陈坚[1,2] 

机构地区：[1]江南大学 工业生物技术教育部重点实验室,无锡214122 [2]食品科学与技术国家重点实验室,无锡214122

出　　处：《工业微生物》2013年第1期41-45,共5页Industrial Microbiology

基　　金：国家自然科学基金重点项目(20836003);973项目(2007CB714306);教育部新世纪优秀人才支持计划(NCET-07-0378);江苏高校优势学科建设工程资助项目资助

摘　　要：为提高过氧化氢酶的应用稳定性和储藏稳定性,选用无机盐、醇类、大分子有机物、糖类、表面活性剂等物质为备选稳定剂,进行不同浓度的单因素实验,然后进行正交实验,又研究了蛋白酶抑制剂对过氧化氢酶酶液中蛋白酶的抑制效果,得到了最佳的稳定剂组合乙酸钠80 g/L,吐温20 2 mL/L,乙二醇80 mL/L,明胶1 g/L,六偏磷酸钠5 g/L,使蛋白酶得到抑制,提高了过氧化氢酶的稳定性。对最佳组合进行了酶的应用稳定性和储藏稳定性实验,在60℃处理15 min,酶活残留率为75.2%提高了43.2%;在常温25℃放置3个月,酶活残留率为90%,提高了67%,酶的应用稳定性和储藏稳定性得到了很大提高,为工业化的生产和应用奠定了基础。In order to improve the stability of catalase during industrial application and production, the effects of inorganic salts, alcohols, organic macromolecules, carbohydrates, surfactants and protease inhibitors on the stability of catalase were investigated. The optimal combination of different stabilizers （sodium acetate 80 g/L, tween 20 2 mL/L, glycol 80 mL/L, gelatine 1 g/L sodium hexametahposphate 5 g/L） was found through orthogonal experiments. Then, the effects of optimal stabilizer combination on CAT activity were compared at 60 ~C for 15 rain and at 25~C for three months, the residual activity rate of 75.2% and 90% were obtained, which were 43.2% and 67% higher than those of the control, respectively. The results demonstrated that the optimal stabilizer combination greatly improved the storage stability and applied stability of CAT, laying the basis for industrial production and application.

关 键 词：过氧化氢酶 稳定剂 应用稳定性 储藏稳定性 

分 类 号：Q55[生物学—生物化学]