响应面法优化Enterobacter sp.SYA2乳糖酶发酵工艺  

Optimization of fermentation process for lactase by Enterobacter sp.SYA2 using response surface methodology

作　　者：张卫兵[1] 文鹏程[1] 刘芳宁[1] 艾对元[1] 贠建民[1] 张炎[1] 梁琪[1] 

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品工业科技》2013年第3期180-184,共5页Science and Technology of Food Industry

基　　金：甘肃省科技重大专项(1002FKDA046);甘肃省农业生物技术专项(GNSW-2010-17);兰州市政研产合作支撑计划项目(2011-1-105)

摘　　要：采用单因素实验对Enterobacter sp.SYA2产乳糖酶条件进行优化,优化后的发酵条件为:培养温度37℃、装液量50mL/250mL、摇床转速175r/min、接种量5%(V/V)、种龄8h,此条件下酶活为9.02U/mL;通过Plackett-Burman设计和Box-Behnken设计对菌株发酵培养基进行了优化,得到的最佳发酵培养基配方为:乳糖31.1g/L、蛋白胨26.0g/L、酵母膏5.0g/L、K2HPO43.0g/L、NaCl6.0g/L、MnCl20.5g/L、pH6.5,优化后的酶活为15.95U/mL。Firstly,fermentation conditions for lactase by Enterobacter sp.SYA2 were optimized by single factor experiment,the result showed that the optimum temperature was 37℃,liquid volume in flask was 50mL /250mL, vorotation speed was 175r /min,inoculation volume was 5% （ V/V） ,seed age was 8 hours.Then Plackett- Burman design and Box-Behnken Design were combined to optimize the medium components of lactase production.The results showed that the optimal medium were as follows：lactose 31.1g /L,peptone 26.0g /L,yeast extract was 5.0g /L, K 2 HPO 4 3.0g /L,NaCl 6.0g /L,MnCl 2 0.5g /L,initiation pH was 6.5. After optimization,the lipase activity was 15.95U /mL.

关 键 词：ENTEROBACTER SP SYA2 乳糖酶 优化 发酵工艺 

分 类 号：TS201.3[轻工技术与工程—食品科学]