羊水细胞原代和传代普通培养方法在产前诊断中应用比较分析  

Analysis different methods between amniotic fluid cells primary culture and passage culture in prenatal diagnosis

作　　者：夏燕[1] 段玲[1] 加米拉[1] 韩锐[1] 韩宁宁[1] 刘志娟[1] 武爽[1] 王晓岚[1] 

机构地区：[1]新疆医科大学第一附属医院产前诊断中心,乌鲁木齐830054

出　　处：《中国优生与遗传杂志》2013年第1期28-29,18,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的通过比较羊水细胞原代和传代普通培养方法,探讨和寻求实现羊水培养成功的方法。方法 77例羊水中35例羊水进行原代普通培养,42例羊水进行传代普通培养。结果 (1)羊水原代普通培养法较羊水传代普通培养法步骤简化,无需进行传代;接种至收获时间缩短(细胞克隆较多时);制片获得羊水染色体较长,染色体形态好,便于分析;但细胞染色体分裂相相对有所减少。(2)羊水细胞克隆较少(<10个克隆)时传代普通培养较原代普通培养法获得较多的细胞染色体分裂相,满足染色体计数和核型分析需要。结论当羊水细胞克隆较多时,羊水原代普通培养法进行羊水细胞染色体产前诊断有明显优势,应优先选择;当羊水细胞克隆较少时,采用羊水传代普通培养法更能保障培养成功。Objective： To investigate a successful amniotic fluid cells culture method,compare different methods between amniotic fluid cells primary culture and passage culture.Methods：35 of 77 amniotic fluid cells with primary culture,42 amniotic fluid cells with passage culture.Results：（1） Primary culture was more simple,we cloud short the time and get the longer chromosomes in primary culture,but got the less chromosomes.（2）We cloud get the more chromosomes to analysis in passage culture,when the number of cell clones was less then 10.Conclusions： We should choose primary culture,when we could get the more cell clones;We should choose passage culture,when we could get the less cell clones.

关 键 词：羊水 普通培养 产前诊断 

分 类 号：R714.5[医药卫生—妇产科学]