基于电沉积石墨烯阪崎肠杆菌免疫传感器研究  

Immunosensor for Enterobacter sakazakii based on electrochemically reduced graphene oxide

作　　者：胡雪[1] 窦文超[1] 赵广英[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,杭州310035

出　　处：《食品科技》2013年第2期296-300,共5页Food Science and Technology

基　　金：国家自然科学基金项目(30571623);浙江省重中之重项目(Z05-178);浙江工商大学研究生科技创新项目(1110XJ1511104)

摘　　要：为研制一种新型免疫传感器并用于快速检测阪崎肠杆菌(E.sakazakii),采用自组装方法将辣根过氧化物酶标记的阪崎肠杆菌抗体(HRP-anti-E.sakazakii)固定于修饰有电沉积石墨烯(ERGO)的丝网印刷碳电极上,制得一次性酶免疫传感器。采用循环伏安法(CV)考察不同修饰电极的电化学特性,根据CV还原峰电流值的变化对E.sakazakii进行快速检测。在优化的检测条件下,该方法检测E.sakazakii的线性范围为102～109cfu/mL,检测限为9.1×101cfu/mL(S/N=3)。该酶免疫传感器灵敏度高,并具有良好的特异性、重现性(RSD=6.4%)、稳定性(4℃无菌容器中放置15d后电流响应为初始值的90.05%),具有一定潜在应用价值。A novel immunosensor based on electrochemically reduced graphene oxide was developed for detection of bacteria,Enterobacter sakazakii.Firstly,a screen-printed carbon electrode（SPCE） was modified by electrochemical method to deposit the graphene.The horseradish peroxidase labeled bacteria-specific antibody（HRP-anti-E.sakazakii） was assembled onto the modified electrode to improve the sensitivity of immunosensor.Cyclic voltammetry（CV） was carried out to characterize the electrochemical properties of different electrodes and detect E.sakazakii.Under optimal conditions,the electrochemical immunosensor for E.sakazakii exhibited a linear range of 10 2 ～10 9 cfu/mL with a detection limit of 9.1×10 1 cfu/mL（S/N=3）.Thus,the novel immunosensor constructed in this study can specifically,sensitively and rapidly detect E.sakazakii,which may contribute to further food-borne pathogen detection.

关 键 词：免疫传感器 电沉积石墨烯 阪崎肠杆菌 快速检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]