耐酸及耐蛋白酶的木聚糖酶产生菌的筛选  

Screening of the Strains of Black Mold Producing Xylanase Stable to Acid and Protease

作　　者：刘新育[1] 李学琴[1] 王明道[1] 姜晨冰[1] 陈红歌[1] 

机构地区：[1]河南农业大学生命科学学院农业部农业微生物酶工程重点实验室,河南郑州450002

出　　处：《河南农业科学》2012年第6期152-154,共3页Journal of Henan Agricultural Sciences

基　　金：河南省科技厅重点攻关项目(0423021600)

摘　　要：通过模拟畜禽胃肠道环境,对保藏的9株木聚糖酶产生菌的粗酶液分别进行酸处理、胃蛋白酶处理以及胰蛋白酶处理,测定木聚糖酶的残余活力,结果发现,多数菌株所产木聚糖酶对酸和胃蛋白酶的稳定性较差,残余酶活力分别在50%以下和20%以下;而不同木聚糖酶对胰蛋白酶的稳定性差异较大,残余酶活力在30%～90%。在所有菌株中,黑曲霉3092和UV11所产木聚糖酶对酸和蛋白酶的稳定性都较好,适合在胃肠道环境中作为饲料添加剂使用。The residue activities of the crude xylanases of nine mold strains were determined after the enzyme preparation were exposed to acid,pepsin and typsin,repectively,simulating the fowl gastrointestinal environment.The results show that there are lower stabilities to acid and pepsin,with less than 50% and 20% of residue xylanase activities,repectively.However,the stabilities of these xylanases to trypsinase are evidently different,with a range from 30% to 90% of residue xylanase activities.Amongst all the strains,Xylanses produced by Aspergillus niger 3092 and Aspergillus niger UV11 were more resistant to both acid and protease,suggesting good performance in the digestive tract as a feed supplement.

关 键 词：木聚糖酶 胃蛋白酶 胰蛋白酶 胃酸 

分 类 号：Q556.2[生物学—生物化学]