一株产α-葡萄糖苷酶菌株的鉴定和选育  

Identification and Breeding of an Alpha-glucosidase Producing Strain

作　　者：吴孔阳[1] 齐宗献[2] 黄桂华[2] 曹喜秀[2] 李秋蓉[2] 陈桂光[2] 梁智群[2] 

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004 [2]广西大学生命科学与技术学院,广西南宁530005

出　　处：《食品研究与开发》2012年第12期145-149,共5页Food Research and Development

基　　金：国家高技术研究发展计划(863计划)(No.2006AA10Z339);国家自然科学基金项目(No.20362001)

摘　　要：通过平板初筛和摇瓶复筛,结合酿酒酵母利用可消化性糖和高效液相色谱法,从海泥中分离到一株产α-葡萄糖苷酶菌株YX41,纯化后经形态观察、生理生化特征和26 S rDNA序列及系统发育树分析,鉴定菌株YX41为伯顿毕赤酵母。通过UV-LiCl对出发菌株YX41进行复合诱变处理,获得一株正向突变株ZG36,该突变株经摇瓶发酵,α-葡萄糖苷酶活力达到4.32 U/mL,比出发菌株提高了32%。Alpha-glucosidase-producing strains were isolated from marine mud by HPLC and Saccharomyces cerevisiae using digestible sugars including glucose,maltose and maltotriose after plate screening and re-screening experiments.The strain was identified as Burtoni Pichia according to its morphological morphological observation,physiological and biochemical characteristics,26 S rDNA sequence analysis and phylogenetic tree analysis.The strain was further complex mutated by UV-LiCl,the higher α-glucosidase-producing mutant strain ZG36 was obtained,which showed α-glucosidase activity of 4.32 U/mL in shaking flask fermentation,about increased 32 % when compared with its parent strain.

关 键 词：Α-葡萄糖苷酶 伯顿毕赤酵母 鉴定 诱变 

分 类 号：TQ925[轻工技术与工程—发酵工程]