大肠菌群测试片中冷水可凝凝固剂初步研究  

PRELIMINARY STUDY ON COLD-WATER-COAGULABLE COAGULATOR FOR COLIFORM COUNT PLATE

作　　者：陈晓珑[1] 董煜[1] 陈灿映[1] 张晓峰[1] 李文杰[1] 

机构地区：[1]郑州大学公共卫生学院,河南郑州450001

出　　处：《河南工业大学学报（自然科学版）》2013年第1期77-81,共5页Journal of Henan University of Technology：Natural Science Edition

基　　金：河南省重大公益科研项目(101100911300)

摘　　要：与国标倾注培养法比对,研究自制冷水可凝凝固剂(XGP)的胶体性能,评价其作为大肠菌群测试片凝固剂的可行性.对XGP的保水性能、pH值、微生物分解利用程度和抑菌效果进行研究,组装成测试片后进行特异性、灵敏度和食品样品检验.研究结果显示,XGP对10种试验菌株均无抑制作用,pH为中性,在短时间(5 d)内不支持微生物生长,不易被分解利用.与国标倾注培养法相比,其具有良好的保水性能(P<0.05),所检出的大肠菌群菌落数、灵敏度及食品样品阳性检出率差异均无统计学意义(P>0.05).XGP具有特异性强、灵敏度高及经济性好等优良特性,可作为培养基载体应用于大肠菌群测试片中.In comparison with the tilt-pour method of the national standard, we studied the colloidal property of a self-made cold-water-coagulable coagulator （XGP） to evaluate the feasibility to serve as the coagulator of a coliform count plate, and also studied the water retention property, pH value, microorganism decomposition and utilization degree and antibacterial effect of XGP as well as the specificity, sensitivity and food sample detection rate after assembling to a count plate. The results showed that XGP was neutral, had no inhibition effect on 10 kinds of test strains, did not support microorganism growth in short time （5 days）, and was difficult to be decomposed. In comparison with the tilt-pour method, the XGP had good water retention property （P〈0.05）, and the coliforms specificity, the sensitivity and the food sample detection rate had no statistical significance （P〉0.05）. Accordingly, the XGP had high specificity, high sensitivity, high economic property, and could be used in the coliform count plate as the medium carrier.

关 键 词：大肠菌群 细菌测试片 冷水可凝凝固剂 快速检测 

分 类 号：TS201.3[轻工技术与工程—食品科学] R155.5[轻工技术与工程—食品科学与工程]