牛初乳对子代雌鼠子宫结构及雌激素受体表达的影响  

Effects of bovine colostrum on the structure of uteri and the expression of estrogen receptor in rat's adult female offsprings

作　　者：徐丽[1] 张兰威[1] 张玉梅[2] 刘钊燕[2] 吕艳丽[2] 

机构地区：[1]哈尔滨工业大学食品科学与工程学院,黑龙江哈尔滨150081 [2]北京大学医学部公共卫生学院,北京100191

出　　处：《中国食品卫生杂志》2013年第1期20-23,共4页Chinese Journal of Food Hygiene

摘　　要：目的研究妊娠期和哺乳期通过SD母鼠暴露于牛初乳及断乳后继续食用牛初乳对成年子代雌鼠子宫发育及雌激素受体(ERα和ERβ)表达的影响。方法将母鼠随机分成2组,牛初乳组和空白对照组。牛初乳组母鼠在交配前期、妊娠期和哺乳期食用含有牛初乳的饲料,仔鼠3周断乳后食用对应于母鼠的饲料至成年。空白组母鼠和仔鼠食用普通饲料。仔鼠成年后处死,取其血液,剥离子宫和卵巢称重,检测血清激素,子宫病理学检查;利用免疫组化法检测子鼠子宫内雌激素受体(ERα和ERβ)的表达。结果牛初乳组子代雌鼠体重、卵巢和子宫脏体比与空白组相比差异无统计学意义(P>0.05),血清中催乳素(PRL)含量显著高于对照组,差异有统计学意义(P<0.05),其余激素含量两组间差异无统计学意义(P>0.05),子宫组织结构无异常表现,子宫内雌激素受体ERα表达量高于空白组,ERβ表达量与空白组相近,但与空白组相比差异均无统计学意义(P>0.05)。结论妊娠期和哺乳期暴露于子代的牛初乳不会对子代雌鼠子宫发育和雌激素受体表达产生不利影响。Objective To study the effects of gestational and lactational exposure to bovine colostrum on the structure of uterus and the expression of estrogen receptor （ERα, ERβ） of the female offspring of Spraque-Dawley rats. Methods SD rats were randomly divided into bovine colostrum and control group and fed before mating and during gestation and lactation with bovine colostrum diet and normal diet. Female offsprings were sacrificed after maturity and uteri and ovaries were removed for weighing. The hormone levels in serum were determined and the uteri were used for pathological examination. Expressions of estrogen receptor α （ERα） and estrogen β （ERβ） in uterus were examined using immunohistochemical method. Results There was no significant difference in the female offsprings＇ body weight and ratio of uterus and ovary weight to body weight between bovine colostrum diet and control diet （ P 〉 0.05 ）. Bovine colostrum diet significantly increased the level of prolactin （PRL） in serum （P 〈 0.05）. Additionally, the development of uterus was normal and the expression of ERα and ERβ had no significant difference between bovine colostrum group and control group （ P 〉 0. 05 ）. Conclusion The gestational and iactational exposure to bovine colostrum did not cause significant influence on the development of uterus and expressions of ERα and ERβ.

关 键 词：牛初乳 雌性仔鼠 生殖和发育 子宫 雌激素受体 内分泌 

分 类 号：R151[医药卫生—营养与食品卫生学]