基于系统发育树的乳铁蛋白相互作用蛋白的预测  

Prediction of lactoferrin interactive proteins based on phylogenetic tree

作　　者：马文丽[1] 庞莉莉[1] 钮冰[1] 郑文岭[1] 黄俊逸[1] 

机构地区：[1]上海大学生命科学学院,上海200444

出　　处：《北京生物医学工程》2013年第1期53-57,共5页Beijing Biomedical Engineering

基　　金：上海市教育委员会科研创新项目(11YZ18);上海市教委分子生理学重点学科建设项目(J50108)资助

摘　　要：目的利用MEGA5.05软件构建乳铁蛋白及与其相互作用蛋白的系统发育树,以进一步寻找与乳铁蛋白相互作用的蛋白质。方法将乳铁蛋白序列及与其相互作用的蛋白质序列以FASTA格式载入MEGA5.05软件,进化树生成算法采用邻接法,校验次数为1000次,其他参数为缺省数值,生成系统进化树。结果与乳铁蛋白相互作用的蛋白质和乳铁蛋白之间有一定的同源关系,与乳铁蛋白相互作用的蛋白质之间也有一定的同源关系。结论通过MEGA5.05软件构建系统发育树,可以预测出乳铁蛋白与肌红蛋白、载脂蛋白D、谷氨酰胺合酶可能存在相互作用。Objective Lactoferrin and its interactive proteins were analyzed based on phylogenesis to further find proteins that could interact with lactoferrin. Methods Lactoferrin sequence and its interactive protein sequences were loaded into software MEGA 5.05 to construct phylogenetic tree in FASTA format with neighbor-joining method, the calibration frequency was 1000 times and other parameters were defaulted. Results First, lactoferrin and its interactive proteins had definite genetic relationship. Second,the interactive proteins themselves might be homologous. Conclusions Interrelation may exist among lactoferrin, myoglobin, apolipoprotein and glutamine synthetase based on phylogenetic tree by MEGAS. 05.

关 键 词：乳铁蛋白 系统发育 蛋白互作 系统进化树 

分 类 号：R318.04[医药卫生—生物医学工程]