重组大肠杆菌利用不同培养基发酵产琥珀酸的研究  

Succinic acid fermentation in different medium conditions by recombinant Escherichia coli

作　　者：华渤文[1] 赵锦芳[1] 王金华[1] 肖子康[1] 赵筱[1] 

机构地区：[1]湖北工业大学生物工程学院,发酵工程教育部重点实验室,湖北武汉430068

出　　处：《食品工业科技》2013年第6期227-230,共4页Science and Technology of Food Industry

基　　金：湖北省科技厅科研项目(2011CDA008;2011CDB076);湖北省教育厅优秀中青年人才项目(Q20121405)

摘　　要：微生物发酵法生产琥珀酸具有广阔的应用前景,实验针对实验室构建的工程菌Escherichia coli WS100(△ldhA、△adhE、△pflB、△poxB、△ackA)在不同培养基中进行厌氧发酵,考察其发酵特性。分别采用NBS培养基和玉米浆培养基,在含4L发酵液的发酵罐中进行补料-分批发酵。发酵83h后,Escherichia coli WS100在玉米浆培养基中消耗葡萄糖96.17g/L,积累琥珀酸63.45g/L,生产强度和质量收率分别为0.76g(/L·h)和65.98%;Escherichia coli WS100在NBS培养基中消耗葡萄糖110.30g/L,琥珀酸的产量达到84.98g/L,生产强度和质量收率分别为1.02g(/L·h)和77.04%。实验结果表明,Escherichia coli WS100在玉米浆培养基和NBS培养基中发酵均有较高的琥珀酸产量,其中在NBS培养基中琥珀酸的产量、生产强度和质量收率均比在玉米浆培养基中高。The production of succinic acid by bacterial fermentation has a great prospect for industrial application, In this study, the fermentative productions of succinic acid by recombinant Escherichia coil WS100 （AldhA, AadhE, ApflB, ApoxB,△ackA） with different media were investigated. 4L NBS medium and corn steep liquor medium were utilized respectively for fed batch fermentations with E.cofi WS100 in fermenters. After 83h of fermentation, 96.17g/L glucose was consumed in corn steep liquor medium,with the production of 63.45g/L succinate. The productivity and yield reached 0.76g/（L·h） and 65.98%, respectively. In NBS medium, 110.30g/L glucose was consumed with the production of 84.98g/L succinate. The productivity and yield reached 1.02g/（L .h） and 77.04%,respectively. The results indicated fine succinate fermentative production was by WS100 in both media, and with higher productive amount, productivity and yield in NBS medium.

关 键 词：琥珀酸 玉米浆 葡萄糖结晶废糖液 大肠杆菌 

分 类 号：TQ921.7[轻工技术与工程—发酵工程]