典型性甲基供体对脱氮假单胞杆菌代谢过程的氧调控作用  

Regulatory effect of the typical methyl-group donors on the oxygen metabolism in Pseudomonas denitrificans

作　　者：周佳[1] 程新[1] 彭志远[1] 彭卫福[1] 李昆太[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西南昌330045

出　　处：《食品工业科技》2013年第7期162-164,168,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金(31060009);江西省自然科学基金(2010GQN0062);江西省教育厅科技项目(GJJ10416);江西农业大学基金项目(2008-2519)

摘　　要：本文在7L发酵罐中分别以甜菜碱和氯化胆碱作为合成维生素B_(12)的甲基供体,分别考察了二者对脱氮假单胞杆菌代谢过程的影响。结果表明:使用氯化胆碱的罐批出现了溶氧过早回升、pH剧烈下降等异常的代谢现象,致使发酵过程的菌体生长缓慢且维生素B_(12)合成量低;而使用甜菜碱作为甲基供体时,其菌体代谢活力要明显优于使用氯化胆碱的罐批,整个发酵过程的pH和溶氧均能保持在稳定且合适的水平,最终的菌体量和维生素B_(12)产量也更高。In this paper, the fermentation processes of Pseudomonas denitrificans were investigated in 7L fermenter by using betaine and choline chloride as the typical methyl- group donors for vitamin Bl2 biosynthesis, respectively.When using choline chloride as the methyl- group donor, the dissolved oxygen （DO）and pH respectively presented an obviously zooming and dropping phenomena, resulting in a slow cell growth and low vitamin B12 biosynthesis.However,the metabolism capability under betaine as methyl-group donor was significantly better than that of choline chloride, and the pH and DO could be maintained at a stable and appropriate level during the whole fermentation processes. As a result, the final cell growth and vitamin B12 production were significantly higher than those obtained in choline chloride.

关 键 词：脱氮假单胞杆菌 维生素B12 甲基供体 代谢过程 

分 类 号：TS201.3[轻工技术与工程—食品科学]