补料培养在猪链球菌疫苗生产中的应用  

Application of Cultivation Methods in Production of Streptococcosis suis Vaccine Strain

作　　者：付强[1,2] 程立坤 苗立中[2] 何军柱 沈志强[2,3] 韩文瑜[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]山东省滨州畜牧兽医研究院,山东滨州256600 [3]山东绿都生物科技有限公司,山东滨州256600

出　　处：《动物医学进展》2013年第3期133-136,共4页Progress In Veterinary Medicine

基　　金：山东省滨州畜牧兽医研究院自主创新基金项目(201002);山东绿都生物科技有限公司技术创新项目(201003)

摘　　要：为了优化猪败血性链球菌弱毒株(ST171)活疫苗的生产工艺,考察不同培养方法对链球菌主要代谢副产物乳酸、活菌数的稳定性以及冻干率的影响,采用批培养、恒速补料及溶氧反馈补料的三种培养策略。结果显示,试验条件下补料培养模式不能降低代谢副产物乳酸的生成量,但恒速补料培养方式能延长活菌数的稳定期,三种培养方式在6h时收获菌液冻干率最高,随培养时间延长,冻干率均降低。应用恒速流加方式进行猪链球菌疫苗生产时,菌体密度、活菌数及冻干率均最高。Three cultivation methods were developed in production of Streptococcosis suis vaccine strain （ST171）, such as batch culture, constant feeding rate strategy and DO-stat feeding strategy. Effects of the three cultivation methods on accumulation of lactic acid, stability of viable count and lyophilized rate were investigated, and results indicated that excretion of by-products could not be decreased with the culti- vation methods in this study and stationary phase of viable count extended by applying constant feeding rate strategy. The high lyophilized rate obtained at 6 h and the lyophilized rate decreased with increasing the cultivation peroid. The constant feeding rate strategy applied in ST171 production, not only maximum optical density and viable counts obtained, but also the lyophilized rate was maximum.

关 键 词：猪链球菌 培养方式 发酵 乳酸 

分 类 号：TQ920.62[轻工技术与工程—发酵工程]