牦牛乳酥油源白地霉S122产脂肪酶培养基的优化  

Medium optimization for lipase produced by Geotrichum candidum S122 isolated from yak butter

作　　者：孙国政 甘伯中[2] 艾对元[2] 张卫兵[2] 

机构地区：[1]庆阳市食品药品检验所,甘肃庆阳745000 [2]甘肃农业大学食品科学与工程学院,甘肃兰州73000

出　　处：《甘肃农业大学学报》2013年第1期135-139,共5页Journal of Gansu Agricultural University

基　　金：兰州市政研产合作支撑计划项目(2011-1-105)

摘　　要：采用Plackett-Burman和中心组合设计相结合的方法,对分离自牦牛乳酥油的菌株白地霉S122产脂肪酶培养基进行了优化.结果表明,蔗糖、硫酸镁和硫酸锰对发酵产酶影响显著(P<0.05);最适的发酵培养基为:蔗糖35.54g/L,蛋白胨30.00g/L,尿素5.00g/L,硫酸镁1.01g/L,硫酸锌0.50g/L,硫酸亚铁0.50g/L,硫酸锰1.02g/L,氯化钙1.00g/L,磷酸氢二钾5.00g/L;利用优化培养基,白地霉S122产脂肪酶酶活达49.5U/mL,比优化前提高了26.92%.Plackett-Burman design and central composite design were combined to optimize the medium components of lipase produced by Geotrichum candidum S122.The results showed that sucrose,magnesium sulfate and manganese sulfate were the most important factors for the production of lipase,and the optimal medium components for lipase production were as follows： sucrose 35.54 g/L,peptone 30.00 g/L,urea 5.00 g/L,magnesium sulfate 1.01 g/L,zinc sulfate 0.50 g/L,ferrous sulfate 0.50 g/L,manganese sulfate 1.02 g/L,calcium chloride 1.00 g/L and dipotassium hydrogen phosphate 5.00 g/L.Using the optimized medium,the lipase activity reached to 49.5 U/mL,which increased by 26.92% than before.

关 键 词：白地霉S122 脂肪酶 牦牛乳 酥油 培养基优化 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]