吉龙草挥发性成分的GC-MS分析  

GC-MS Analysis of Volatile Compounds of Elsholtzia communis

作　　者：芦燕玲[1,2] 黄静[1] 徐世涛[1] 高则睿[1] 施红林[1] 李忠[1] 

机构地区：[1]云南烟草科学研究院,昆明650106 [2]云南民族大学云南省化学实验教学示范中心/国家民委--教育部共建民族药资源化学重点实验室,昆明650500

出　　处：《中国药房》2013年第15期1403-1406,共4页China Pharmacy

基　　金："云南特色天然烟用香原料开发及其应用"项目资助(No.2009FL01)

摘　　要：目的:分析吉龙草的挥发性成分。方法:分别建立了同时蒸馏萃取和顶空加热直接进样的气相色谱-质谱(GC-MS)联用技术对吉龙草的挥发性成分进行分析,并探讨了蒸馏温度和料液比对吉龙草得油率的影响。结果:从吉龙草中共分离鉴定了121个挥发性成分,主要成分为柠檬醛、乙酸等;在蒸馏萃取时间为5h、料液比为1:30(m/V)的条件下,其得油率为2.40%,优于其他条件组合。结论:采用水蒸气蒸馏GC-MS联用技术(文献报道)、同时蒸馏萃取GC-MS联用技术、顶空加热GC-MS联用技术3种方法检出的挥发性成分在类别与含量方面都具有各自的侧重点。同时蒸馏萃取法提供的吉龙草挥发物信息比水蒸气蒸馏法、顶空加热法提供的信息更丰富。建议若以GC-MS指纹图谱表征吉龙草的挥发性成分,应结合3种方法采集的信息。OBJECTIVE： To analyze volatile compounds of Elsholtzia communis. METHODS： Simultaneous distillation and extraction （SDE）-GC-MS and Head-space injection（HS）-GC-MS technology were set up to analyze volatile compounds in E. commuhis, and the influence of steaming temperature and solid-liquid ratio on the essential oil yield of E. communis were discussed. RESULTS： 121 volatile compounds were isolated and identified from E. communis, and the main components were citral, acetic acid, etc. Besides, the yield of essential oil was 2.40% on the condition that steaming time was 5 hours, solid-liquid ratio was 1：30（m/V）, which better than others. CONCLUSION： The information about volatile compounds in E. communis obtained by SD from literature reports, SDE, HS have different focus. SDE is more plentiful than obtained by SD or HS analysis. We can get a better representation system for GC-MS fingerprints of volatile compounds in E. communis when combine these methods together.

关 键 词：吉龙草 挥发性成分 同时蒸馏萃取法 顶空加热法 气相色谱-质谱联用技术 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]