响应面优化琼脂糖的EDTA-Na_2法提取工艺  

EDTA-Na_2 Extraction of Agarose with Response Surface Optimization

作　　者：李龙[1,2] 苏永昌[2] 吴成业[2] 

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]福建省水产研究所,福建厦门361013

出　　处：《福建农业学报》2013年第2期158-165,共8页Fujian Journal of Agricultural Sciences

基　　金：国家海洋公益性行业科研专项(201205022-4)

摘　　要：探讨了提取工艺中主要影响因子EDTA-Na2浓度、提取温度、提取时间对硫酸根含量及凝胶强度的影响,并利用响应面法优化提取工艺,得出最佳工艺参数为:EDTA-Na2浓度4.5g.L-1,提取温度65℃,提取时间3h,在最佳提取工艺的条件下制得的琼脂糖,其硫酸根含量为0.48%,凝胶强度为1 073.09g.cm-2。对最优工艺参数进行重复性验证,相对误差在1.0%以内。表明该方法具有较高的准确性和重复性,可作为琼脂糖的粗提方法。This paper studied the technique of agarose extraction with EDTA-Na2 method.The main factors discussed were： EDTA-Na2 concentration,extraction temperature and extraction time on influencing in sulfate radical content and gel strength.Using the response surface method to optimize the extraction process,the best parameters were EDTA-Na2 concentration 4.5 g·L-1,extraction temperature 65℃ and extracting time 3 h.With the optimized parameters,the sulfate radical content and gel strength of agarose extracted were 0.48% and 1 073.09 g·cm-2,respectively.The optimal extraction parameters were verified by repetitive tests with relative error 1.0%.It is suggested that EDTA-Na2 method is accurate and repeatable in using for agarose extraction.

关 键 词：响应面法 EDTA-Na2法 琼脂糖 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]