Fmrl基因敲除小鼠脑组织神经调节蛋白1表达的改变及其意义  

Expression of neuregulin 1 and its significance in brain tissues of FMRI gene knockout mice

作　　者：卢韬[1] 欧阳梅[1] 周林涛[1] 易咏红[1] 

机构地区：[1]广州医学院第二附属医院神经内科,广州医学院神经科学研究所,广州510260

出　　处：《中华神经医学杂志》2013年第4期339-342,共4页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金重点项目（04105429）

摘　　要：目的明确神经调节蛋白1（NRGl）在Fmrl基因敲除（K0）小鼠中的变化，探讨其在脆性x综合征发病机制中的作用。方法应用免疫组织化学染色法检测FVB近交系雄性2周龄FrarlKO小鼠（K02w）、4周龄FmrlKO小鼠（K04w）和同龄野生型（WT）／J,鼠大脑皮层及海马CAl区、CA3区、齿状回中神经调节蛋白1的阳性神经元的数量；Westemblotting检测上述小鼠大脑皮层和海马组织NRGl蛋白的含量。结果与同龄wT小鼠相比，K0m、K04w小鼠大脑皮层、海马CAl和CA3区NRGl阳性神经元的数量明显减少，在海马齿状回却明显增多，差异均有统计学意义（氏O．05）；KOw、K04w小鼠大脑皮层、海马中NRGl含量（相对分子质量为55000亚型）分别较同龄的wT小鼠明显减少，差异亦有统计学意义（氏0．05）。结论砌rJKO小鼠大脑皮层和海马组织NRGl阳性神经元及NRGl蛋白表达明显减少，NRGl可能参与脆性X综合征发病机制。Objective To explore the expression changes ofneuregulin 1 （NRG1） in Frnrl gene knockout （Fmrl KO） mice, and its possible role in fragile X syndrome pathogenesis. Methods Immunohistochemistry and Western blotting were simultaneously employed to detect the expression levels of NRG1 in the hippocampus and the cortex of FVB strain Fmrl KO mice and wild type （WT） controls at the age of 2 and 4 weeks. Results The number of NRGl-positive cells in the CA1 and CA3 regions of hippocampus and the cortex of Fmrl KO mice at the age of 2 and 4 weeks was significantly smaller than that in the age-matched WT mice （P〈0.05）. The NRG1 protein levels in the hippocampus and cerebral cortex of Fmrl KO mice at the age of 2 and 4 weeks were also decreased as compared with those in the age-matched WT mice （P〈0.05）. Conclusion The number of NRGl-positive cells and NRG1 protein levels in the CA1 and CA3 regions of hippocampus and the cortex ofFmrl KO mice are decreased, indicating that NRG1 might involve in the pathogenesis of Fragile X syndrome.

关 键 词：脆性X综合征 神经调节蛋白1 基因敲除 

分 类 号：R74[医药卫生—神经病学与精神病学]