潲水油中辣椒素检测技术研究  

Research on detection technology of capsaicinoids in hogwash oil

作　　者：毛丽莎[1] 仲岳桐[1] 卢紫燕[1] 康莉[1] 刘桂华[1] 邓平建[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国卫生检验杂志》2013年第3期544-546,550,共4页Chinese Journal of Health Laboratory Technology

基　　金：深圳市科技计划项目(201202094);深圳市战略性新兴产业发展专项资金"05-地沟油快速检测检验技术研究"项目

摘　　要：目的:建立高灵敏度的潲水油中辣椒素的测定的方法,用于检测甄别潲水油。方法:样品用0.5 mol/L的氢氧化钠溶液振荡提取,反相C18小柱浓缩净化,以乙腈(0.01%甲酸)-0.01%甲酸水溶液为流动相进行梯度洗脱,通过液相色谱-质谱/质谱以MRM方式检测,以二苯胺为内标定量。结果:测定辣椒碱、二氢辣椒碱方法的线性范围均为0.2μg/L～40.0μg/L,线性相关系数分别为0.9997和0.9999,检出限分别为3.2 ng/kg、2.8 ng/kg,回收率分别为87.2%～90.6%、91.9%～102.4%。对39个油样进行检测,对潲水油的鉴别判断正确率为92.3%。结论:方法简便,灵敏度好,选择性好,可作为潲水油的有效鉴别手段。Objective：To develop a method for determination of capsaicin in hogwash oil with high performance liquid chromatography - mass spectrometry. Methods： The oil sample was extracted by shaking 0.5 mol/L Sodium hydroxide solution and then cleanup was performed on C18 cartridge. The gradient elution was carried out with acetonitrile （0.01% formic acid） -0.01% formic acid as the mobile phase for the baseline separation of the two capsaicinoids in thirty minutes. Diphenylamine was used as internal standard and Multiple Reaction Monitor （MRM） method was applied for detection. Results： The linear range of the two capsaicinoids were all found to be 0.2 μg/L 40.0 μg/L, and the correlation coefficients of the standard curve were 0. 9997 and 0. 9999. The limits of detection were 3.2 ng/kg and 2.8 ng/kg. The recoveries of capsaicin for the spiked samples ranged from 87, 2% to 90.6% and for dihydrocapsaicin ranged from 91.9% - 102.4%. The method was applied for detection of 39 samples and the correct rate was 92.3%. Conculsion ： The HPLC - MS/MS method is suitable for detection of capsaicin and dihydrocapsaicin in hogwash oil and it was proven to be high sensitive and anti -interference.

关 键 词：潲水油 辣椒碱 二氢辣椒碱 液相色谱-质谱 质谱法 

分 类 号：R155.58[医药卫生—营养与食品卫生学]