超高效液相色谱法测定食品中5种天然儿茶素  

Determination of five kinds of natural catechins in foods by ultra performance liquid chromatography

作　　者：雷云[1] 戴明 欧阳利群 郑小严 林雅静[1] 

机构地区：[1]福建医科大学药学院药物分析系,福州350004 [2]福建省产品质量检验研究院国家加工食品质量监督检验中心,福州350002

出　　处：《海峡预防医学杂志》2013年第1期1-3,6,共4页Strait Journal of Preventive Medicine

基　　金：福建省自然科学基金(No.2009J05115);福建省卫生厅青年基金(No.2010-1-34)

摘　　要：目的建立4类食品中5种天然抗氧化剂儿茶素类化合物(GCG、EC、ECG、EGC和EGCG)的超高效液相色谱-二极管阵列检测方法(UPLC-PDA)。方法样品经液液萃取、离心和浓缩后,用超高效液相色谱分离检测。结果本法对5种儿茶素均能在11min内很好分离,检测限仅0.2～2.5μg/g;空白样品的加标回收率77.7%～113.2%,相对标准差为1.1%～8.1%,峰面积在0.1～200μg/mL,呈良好的线性关系,决定系数R2均大于0.99。结论超高效液相色谱法分离速度快,且检测的灵敏度高于传统方法。Objective To establish a method for detecting 5 kinds of natural phenolic antioxidants catechins（GCG,EC,ECG,EGC and EGCG） from 4 kinds of foods by UPLC-PDA.Methods The analytes were extracted from the foods,followed by centrifugation,concentration and detection by UPLC.Results The five kinds catechin and the levels were identified and determined by.UPLC-PDA in 11 minutes,the detection limits were 0.2-2.5 μg/g only.The recovery rates of catechins,spiked blank samples were ranged from 77.7% to 113.2%,and relative standard deviations were 1.1%-8.1%.There was a good linear corrlation between the peak area and correlation of caichins with the peak area 0.1-200 μg/ml,the coefficient of determination（R2） was 0.99 or more.Conclusion UPLC method shows high separation speed and high sensitivity,comparing to common method.

关 键 词：食品检测 超高效液相色谱 儿茶素 

分 类 号：R155.5[医药卫生—营养与食品卫生学] O657.7[医药卫生—公共卫生与预防医学]