海带多糖和茶多酚对鼻咽癌细胞HONE1和CNE2增殖的影响  

The effects of tea polyphenols and laminaria japonica polysaccharides on nasopharyngeal carcinoma cell HONE1 and CNE2

作　　者：孙文忠[1] 魏媛媛[1] 曾曼丽[1] 郑实兴[1] 徐志文[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉-头颈外科,南宁530021

出　　处：《临床耳鼻咽喉头颈外科杂志》2013年第8期425-428,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广西科学基金资助项目(No:桂科自0728241);广西卫生厅重点课题基金资助项目(No:桂卫重200634)

摘　　要：目的:观察海带多糖(LJP)和茶多酚(TP)单独及联合应用对鼻咽癌(NPC)细胞HONE1和CNE2增殖的影响,了解LJP对体内移植瘤的抑制作用,探讨其抗癌机制。方法:以NPC细胞HONE1和CNE2为研究对象,绘制两者生长曲线,计算倍增时间确定对数生长期细胞,采用MTT检测LJP和TP单独及联合应用对两组细胞的增殖抑制率,运用Annexin V加PI双染法检测LJP对HONE1细胞凋亡的影响;以人NPC细胞株HONE1建立裸鼠皮下移植瘤模型及行体内抑瘤实验。结果:LJP和TP均对两组细胞有增殖抑制作用,LJP和TP联合应用对两组实验细胞抑制作用均高于两者单独应用的效果,且这种作用呈浓度依赖关系。流式细胞仪检测结果显示,LJP具有诱导HONE1细胞凋亡的作用,凋亡率随着LJP浓度的增加而增高,320mg/L的凋亡率为(49.51±1.89)%(P<0.01)。体内实验中,25.0mg/kg LJP组、50.0mg/kg LJP组的抑瘤率分别为33.7%(P<0.05)和47.0%(P<0.01),具有明显抑制移植瘤的作用,而12.5mg/kg LJP组的抑瘤率仅为16.4%(P>0.05)。结论:LJP和TP对鼻咽癌细胞HONE1和CNE2增殖均有抑制作用,两者联合应用更具有显著效果,LJP作用机制可能是通过诱导癌细胞凋亡而实现的。Objective：To explore the effects of laminaria japonica polysaccharides（LJP） and tea polyphenols（TP） on nasopharyngeal carcinoma（NPC） cells HONE1 and CNE2,and further to explore the underlying mechanism of antitumor activity of LJP on NPC cell in vivo.Method：To identify the logarithmic growth phase of NPC cells HONE1 and CNE2 through cell growth curve and doubling time by means of MTT,then inhibition of the cells proliferation were detected with LJP and TP separately and combined.With LJP treatment,cell apoptosis of HONE1 was examined by double staining assay.A tumor model,established by subcutaneously inoculation of NPC cell HONE1 into nude mice,was used to evaluate the inhibitory effect of LJP in vivo.Result：Both LJP and TP had inhibition effect on two groups of cell proliferation,and LJP and TP combined effect of inhibition were higher than the two separate on two sets of experimental cell proliferation,whose effect was concentration-dependent.LJP could induce apoptosis of HONE1.With the increasing concentration of LJP,apoptosis rate increased.The apoptosis rate was（49.51±1.89）%（P0.01） when treated with 320 mg/L LJP.The inhibition rate was between 50% to 60% at 72 h after treatment with 320 mg/L LJP.Compared to control group,the growth of xenografts in nude mice was significantly suppressed after administration of LJP at a dose-dependent manner.The inhibition rates were 33.7%（P0.05）and 47.0%（P0.01） when treated with 25.0 mg/kg and 50.0 mg/kg respectively.Whereas the inhibition rate of 12.5 mg/kg group was only 16.4%（P0.05）.Conclusion：LJP and TP can inhibit the proliferation of NPC cells HONE1 and CNE2 respectively,and combined use has a significant effect.LJP can inhibit the growth of NPC probably by inducing apoptosis of NPC cells in vitro and in vivo.

关 键 词：海带多糖 茶多酚 HONE1细胞 CNE2细胞 四甲基偶氮唑蓝检测法 鼻咽肿瘤 

分 类 号：R739.63[医药卫生—肿瘤]