紫外分光光度法在没食子酸体外还原Fe^(3+)能力检测中的应用及其意义  

Application of UV spectrophotometer in detection of ability of gallic acid to restore Fe^(3+) in vitro

作　　者：伍翔群[1] 于兰[2] 于杰[3] 黄彩婷[1] 许维国[1] 牛凤兰[1] 

机构地区：[1]吉林大学公共卫生学院卫生检验学教研室,吉林长春130021 [2]长春中医药大学附属医院脑病科,吉林长春130021 [3]吉林大学中日联谊医院电诊科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2013年第2期414-418,共5页Journal of Jilin University：Medicine Edition

基　　金：国家重点基础研究发展计划(973计划)资助课题(2011CB935800);吉林省科技厅重点项目资助课题(20100905);吉林省高技术产业发展项目资助课题(2010014)

摘　　要：目的:测定没食子酸(GA)体外还原Fe3+的能力,并与公认的抗氧化剂抗坏血酸(Vc)比较,探讨其体外抗氧化性,为GA抗氧化应用提供实验依据。方法:采用不同浓度的GA(0.04、0.08、0.12和0.16g.L-1)和Vc(0.04、0.08、0.12和0.16g.L-1)在邻二氮菲-Fe2+体系发生Fenton反应,在铁氰化钾-Fe3+体系发生Fe3+还原反应,用紫外分光光度计分别检测反应后溶液的吸光度(A)值,应用SPSS 17.0软件包采用双因素方差分析溶液A值和GA及Vc浓度的关系。结果:在邻二氮菲-Fe2+体系中,同浓度的GA与Vc比较,GA对Fe3+的还原能力强于Vc(P<0.05);与0.04g.L-1 GA比较,0.08、0.12和0.16g.L-1 GA对Fe3+还原能力逐渐增强(P<0.05);且乙醇对该体系的A值有影响。在铁氰化钾-Fe3+体系中,当GA与Vc浓度均为0.04g.L-1时,GA对Fe3+还原能力强;而其余浓度下其还原能力均弱于Vc(P<0.05)。当盐酸浓度大于0.84×10-2 mol.L-1时,其对铁氰化钾-Fe3+体系的A值有影响。结论:与Vc比较,GA在体外环境对Fe3+有较强还原能力。Objective To detect the ability of gallic acid（GA） to restore Fe3＋ in vitro,and explore its antioxidant activity in vitro and to compare it with recognized antioxidant ascorbic acid（Vc）,and to provide experimental basis for antioxident application of GA.Methods Different concentrations of GA（0.04,0.08,0.12 and 0.16 g·L-1） and Vc（0.04,0.08,0.12 and 0.16 g·L-1） were used in the phenanthroline-Fe2＋ system to occurre Fenton reaction and the potassium ferricyanide-Fe3＋ system to occurre Fe3＋ restore reaction.The A values of the solution after reaction were detected with UV spectrophotometer.SPSS 17.0 package and two-way ANOVA were used to analyze the relationship between the A values and concentrations of GA and Vc solution.Results In the phenanthroline-Fe2＋ system,the A value of GA solution was larger than that of Vc at the same concentration（P0.05）.Compared with 0.04 g·L-1 GA group,the A values in 0.08,0.12,and 0.16 GA groups were gradually increased（P0.05）;and ethanol influenced the A value of the phenanthroline-Fe2＋ system.In potassium ferricyanide-Fe3＋ system,the Fe3＋ reduction ability of GA was stronger than Vc when their concentrations were 0.04 g·L-1;the abilities were weaker in the other concentrations.When the concentration of HCl was higher than 0.84× 10-2mol·L-1,it had great influence in the A value of potassium ferricyanide-Fe3＋ system.Conclusion Compared with Vc,GA has a stronger Fe3＋ restoring capacity under certain conditions in vitro.

关 键 词：没食子酸 抗坏血酸 邻二氮菲-Fe2+体系 铁氰化钾-Fe3+体系 分光光度比色法 

分 类 号：R914.4[医药卫生—药物化学]