血红密孔菌Pycnoporus sanguineus SYBC-L12固态发酵水稻秸秆产漆酶及其对染料的脱色作用  

Laccase Production Under Solid-State Fermentation of Rice Straw by Pycnoporus sanguineus SYBC-L12 and Its Decolorization of Synthetic Dyes

作　　者：孙啸[1] 蔡宇杰[1] 廖祥儒[1] 张峰[1] 刘家扬[1] 袁小曼[1] 马德丽[1] 席艳茹[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122

出　　处：《食品与生物技术学报》2013年第3期240-249,共10页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2010AA101501);重大科技支撑与自主创新专项引导资金项目(BY2010117);江苏高校优势学科建设工程资助项目;无锡市科技支撑计划社会发展项目(CSE01N1210)

摘　　要：血红密孔菌Pycnoporus sanguineus SYBC-L12能以水稻秸秆作为底物和支撑物进行固态发酵并且具有较高的产漆酶能力。以水稻秸秆为基本培养基,漆酶活力为指标,对产酶培养基和培养条件进行了单因素优化,优化后的漆酶活力约为优化前的10倍。所得漆酶在常温下对蒽醌类染料、三苯甲烷类染料、亚胺醌类染料具有较强的脱色作用,其混合物也可被漆酶大部分脱色,在较高温度下,漆酶可将偶氮类染料部分脱色。此外,科学试验使用的工业废水样品中常见金属离子大部分对脱色作用没有抑制。因此,漆酶作为一种环境友好型生物催化剂,对工业污水的处理具有广阔的应用前景。Pycnoporus sanguineus SYBC-LI2 has strong ability to produce high level of laccase using rice straw as substrate and support materials under solid-state fermentation. We optimized the condition tot laccase production using one-factor-at-a-time method and the laccase activity of the optimized condition was about 10 times higher than that of the original level. The obtained laeease was used to decolorize anthraquinone dyes,triphenyhnethane dyes and ethyleniminoquinone dyes and their mixed one at room temperature,which showed desirable results. Under higher temperature, azo dyes were partly decolorized. Moreover, most mental ions of industrial effluent used in the essay had no inhibitory effect on the deeolorization except a few. Therefore,laccase as a kind of environmentally friendly bio-catalyzer has a great potential in sewage disposal and effluent treatment.

关 键 词：漆酶 水稻秸秆 固态发酵 合成染料 脱色 血红密孔菌 Pycnoporus sanguineus 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]