高效液相色谱法定量分析茶籽粕中的茶皂素  

Quantitative Determination of Tea Saponin in Tea Seed Meal by High Performance Liquid Chromatography

作　　者：张海龙[1] 张维农[1] 胡志雄[2] 齐玉堂[1] 龚任[3] 

机构地区：[1]武汉工业学院食品科学与工程学院,湖北武汉430023 [2]武汉工业学院分析测试中心,湖北武汉430023 [3]国家粮食局武汉科学研究设计院,湖北武汉430070

出　　处：《食品科学》2013年第8期153-156,共4页Food Science

基　　金：国家"863"计划项目(2010AA023003);武汉工业学院食品营养与安全研究院重大项目培育专项(2011Z02)

摘　　要：以脱脂冷榨茶籽饼粕为原料,采用反相ODS填料纯化制备茶皂素标准品,并采用紫外吸收光谱、红外光谱及质谱对该标准品进行鉴定;采用自制标准品为标样,建立一种高效液相色谱法定量分析茶籽饼粕中茶皂素含量的方法。高效液相色谱法条件为反相ODS色谱柱、检测波长215nm、柱温室温、流速1.0mL/min、流动相以乙腈-水体系进行梯度洗脱(0～5min内乙腈体积分数保持为20%,5～10min内流动相乙腈体积分数从20%增至100%,10～15min内流动相乙腈体积分数从100%回至20%)。采用该条件检测得到的茶皂素峰型对称,保留时间合适,杂峰和茶皂素峰分离度较高。In this work, tea saponin was purified by reversed-phase ODS-A column chromatography from degreased cold- pressed tea seed meal for use as a reference standard in the quantitative determination of tea saponin by HPLC and identified by UV absorption spectrometry, FTIR and mass spectrometry. HPLC separation was achieved with reversed-phase ODS column and acetonitrile-water as eluent （an initial acetonitrile concentration of 20% held for 5 min, then increased to 100% in 5 min, and finally decreased back to the initial value） at a flow rate of 1.0 mL/min in gradient mode. UV detection was carded out at 215 nm. Under these conditions, symmetric peaks, desired retention time and good separation between tea saponin and impurity were observed.

关 键 词：高效液相色谱法 茶籽粕 茶皂素 检测 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程]