水解乳清蛋白对辐射损伤大鼠蛋白质营养状态的影响  

Influence of hydrolyzed whey protein intaking on protein nutrition status of rats in radiation injury

作　　者：孙海岚[1] 何秀梅[1] 林欣[1] 邓鑫[1] 刘洁[1] 杨剑[1] 蒋宝泉[1] 

机构地区：[1]第三军医大学第三附属医院野战外科研究所营养科,重庆400042

出　　处：《检验医学与临床》2013年第8期919-920,922,共3页Laboratory Medicine and Clinic

基　　金：重庆市科学技术委员会重点攻关项目(2011AB5038)

摘　　要：目的探讨水解乳清蛋白对辐射损伤大鼠蛋白质营养状态的影响。方法 30只清洁级SD大鼠(雌雄各半),随机分为健康对照组、实验对照组、实验组,每组10只,实验对照组和实验组动物给予一次性6Gray剂量的γ射线全身辐照,实验组饲料氮源为水解乳清蛋白,正常对照组、实验对照组饲料氮源为乳清蛋白,主要观察指标为大鼠摄食量与体质量变化量、粪氮测定与蛋白质表观消化率(AD)、血清清蛋白(Alb)和肠黏膜HE染色等。结果实验组、实验对照组动物辐照后摄食量均明显低于健康对照组,并且体质量均出现下降,但实验组动物9d后体质量开始递增,实验结束时实验组与健康对照组体质量变化量明显高于实验对照组和健康对照组(P=0.017);实验组与实验对照组的粪氮排出量、AD值相比,差异均有统计学意义(P<0.01);实验组和实验对照组大鼠血清ALB含量显著低于健康对照组,但实验组显著高于实验对照组(P=0.041);实验各组肠黏膜HE染色对比明显。结论水解乳清蛋白有利于改善辐射损伤大鼠氮代谢和机体蛋白质营养水平。Objective To compare the influence of hydrolyzed whey protein intaking on nitrogen metabolism of rats in radiation injury. Methods Thirty SD rats were randomly divided into three group., normal control group （n= 10） ,experimental control group （n= 10） and experimental group （n= 10）. Rats in experimental group were fed with hydrolyzed whey protein as nitrogen source, and in normal control group and experimental control group, rats were fed with whey protein as the nitrogen source. Changes of body weights and food-intake were observed, and apparent digestibility（AD） was calculated. The serum albumin（ALB） was tested. The differece of intestinal mucosa was de- tected by hematoxylin-eosin staining in each group. Results The body weights change of experimental group were significantly higher than experimental control group（P=0. 017）. The AD and ALB of experimental group were also significantly higher than experimental control group. Conclusion Application of hydrolyzed whey protein will help to improve the level of body proteina and the nitrogen metabolism radiation injury in rats of rats in radiation injury.

关 键 词：水解乳清蛋白 乳清蛋白 辐射损伤 氮代谢 蛋白质表观消化率 

分 类 号：R-332[医药卫生]