邻苯二甲酸二异丁酯对小鼠主动回避行为及脑组织氧化应激的影响  

Effect of diisobutyl phthalate on active avoidance behavior and oxidative stress of brain tissue in mice

作　　者：马宁[1] 高芃[1] 曹佩[1] 王小丹[1] 徐海滨[1] 

机构地区：[1]国家食品安全风险评估中心,卫生部食品安全风险评估重点实验室,北京100022

出　　处：《中国食品卫生杂志》2013年第2期121-124,共4页Chinese Journal of Food Hygiene

基　　金：国家科技支撑计划课题(2006BAK02A07)

摘　　要：目的对邻苯二甲酸二异丁酯(DiBP)影响小鼠主动回避学习记忆行为进行初步研究,并探讨其是否可引起脑组织氧化损伤。方法将60只昆明小鼠(实验动物房内适应3 d)按体重随机分对照组和4个不同剂量的DiBP试验组(4组剂量分别为50、250、500和1 000 mg/kg BW)。其中,对照组给予玉米油溶剂灌胃,而各试验组给予相应剂量的DiBP玉米油溶液灌胃,实验连续进行8周,普通饲料喂饲,自由饮水。试验结束后,分别对各组动物进行穿梭箱行为学测试,并测定脑组织超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及8-羟基脱氧鸟苷(8-OHdG)的含量。结果 500和1 000 mg/kg BW组与对照组比较,条件反射潜伏期(CL)延长,差异均有统计学意义(P<0.05);1 000 mg/kg BW组与对照组比较,条件反射次数减少,差异有统计学意义(P<0.01);500和1 000 mg/kg BW组与对照组比较,条件性回避率降低,差异均有统计学意义(P<0.05)。各试验组小鼠脑组织SOD的活性与对照组比较差异无统计学意义(P>0.05),但GSH-Px活性呈现降低趋势,250和1 000 mg/kg BW组与对照组比较,酶活性差异有统计学意义(P<0.05);各试验组与对照组相比,8-OHdG含量增加,差异具有统计学意义(P<0.05)。结论 DiBP可损伤实验小鼠主动回避学习记忆能力,并能够导致试验小鼠脑组织发生不同程度的氧化应激损伤。Objective To give a preliminary research on diisobutyl phthalate （DiBP） on active avoidance behavior, and the potential of free radical oxidation damage in brain tissues of mice. Methods Accommodating 60 Kunming mice to the animal room for 3 days, then dividing the mice into 5 groups according to their weights including one control group and four experimental groups were 50, 250, 500 and 1 000 mg/kg BW. The mice were fed with corn oil in control group, and the other groups were fed with different dose of diisobutyl phthalate mixture by garages last for 8 weeks. At the end point, active avoidance response was examined, then all mice were sacrificed and biomarkers in the brains, such as SOD, GSH- Px and 8-OHdG were testeds. Results In the active avoidance response test, the mice of groups 500 and 1 000 mg/kg BW showed longer response latency than control group （P 〈 0.05） and the mice of group 1 000 mg/kg BW showed fewer conditioned response than control group （P 〈 O. 01 ） ; the mice of groups 500 and 1 000 mg/kg BW showed a lower conditioned response rate than control group （ P 〈 0.05） ; the GSH-Px activities in groups 250 and 1 000 mg/kg BW significantly decreased （P 〈 0.05 ）, and the 8-OHdG content increase significantly comparing to the control group, （ P 〈 O. 05 ）. Conclusion Exposure to DiBP could decrease active avoidance ability. Oxidative stress could be induced by DiBP in mice brain tissue.

关 键 词：塑化剂 邻苯二甲酸二异丁酯 主动回避 氧化应激 食品安全 

分 类 号：R114[医药卫生—卫生毒理学]