咖啡酸衍生物对自由基致DNA氧化损伤的联合保护作用  

Protective effect of Caffeic acid derivatives against free radical induced DNA damage

作　　者：李瑞锐[1] 尹文琴[1] 李磊[1] 王丽[1] 陈丽君[1] 戴金凤[1] 

机构地区：[1]南京医科大学公共卫生学院,江苏南京211166

出　　处：《食品工业科技》2013年第10期149-151,174,共4页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(81072338);江苏省优势学科建设项目资助

摘　　要：目的:探讨咖啡酸衍生物(CADs):绿原酸(Chlorogenic acid,ChA)、阿魏酸(Ferulic acid,FeA)、迷迭香酸(Rosmarinic acid,RoA)对自由基致DNA损伤的联合抑制保护效果。方法:运用DPPH自由基及羟自由基反应模型观察不同组成的CADs对自由基的淬灭效应,以CuSO_4-Phen-VitC-H_2O_2-DNA化学发光体系测定不同成分的多酚类物质对·OH致DNA损伤的抑制作用。结果:在30min时间内,三种CADs联合组在浓度为25、50、100、200μg/mL时,对DPPH·清除率分别为28.93%、58.39%、83.93%和84.09%;对·OH清除率分别为33.43%、55.27%、71.23%、77.49%。在DNA化学发光体系中,在25～200μg/mL范围内,三种CADs联合组对DNA损伤产物发光抑制率为12.49%～81.09%。结论:CADs能有效清除DPPH自由基和羟自由基,抑制羟自由基引发的DNA损伤程度,并延迟其受损伤的时间。在各实验组中三种CADs联用组在对DPPH自由基清除率、DNA损伤产物发光抑制率以及保护DNA损伤的能力均最强,体现出协同作用。Objective：Free radical models and a microplate reader were used to explore the scavenging activity of Caffeic acid derivatives against hydroxyl and DPPH free radicals. Methods=The CuSO4-Phen-Vo-H202-DNA chemiluminescences system was employed to determine the inhibitory effect of ChA,FeA and RoA on hydroxyl radical-induced DNA damage. Meanwhile,the protective effect and possible mechanisms of the three phenolics compounds with effective concentration against free radical-induced DNA damage were also explored. Results ：The DPpHo scavenging ratio was 28.93% ,58.39% ,83.93% and 84.09% ,respectively when the CADs concentration was at the 25,50,100,2001Jg/mL within 30rain reaction. The clearance of .OH were 33.43%, 55.27%,71.23%,77.49%. The inhibition ratio of the chemiluminescence of DNA damage product were 12.49% -81.09%. With different CADs concentrations of 25-200tJg/mL,and the time of DNA damage was delayed. Conclusion：CADs could scavenge DPPH~ and .OH obviously and restrain the DNA injury caused by the free radical,or delay the DNA damage time. The scavenging free radical and protective effect of CADs on DNA damage was related to its concentration. The effect was the strongest on DPPH scavenging,the luminescence inhibition rate of DNA damage product and the ability to protect the DNA damage was three CADs joint group,reflect a certain synergy.

关 键 词：咖啡酸衍生物 自由基 DNA损伤 协同作用 化学发光 

分 类 号：TS201.1[轻工技术与工程—食品科学]